107929

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87820..88345 Replicon plasmid p2017C-4173W12
Accession NZ_CP030770
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DSB71_RS28620 Protein ID WP_001159871.1
Coordinates 87820..88125 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DSB71_RS28625 Protein ID WP_000813634.1
Coordinates 88127..88345 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS28585 83247..84044 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
DSB71_RS28590 84106..84357 + 252 Protein_106 DUF4113 domain-containing protein -
DSB71_RS28595 84362..84754 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
DSB71_RS28600 84759..85730 - 972 WP_001103694.1 hypothetical protein -
DSB71_RS28605 85959..86603 + 645 WP_000633911.1 AAA family ATPase -
DSB71_RS28610 86597..86872 + 276 WP_000239529.1 hypothetical protein -
DSB71_RS28615 87010..87819 - 810 WP_000016979.1 site-specific integrase -
DSB71_RS28620 87820..88125 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DSB71_RS28625 88127..88345 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DSB71_RS28635 90667..91308 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / sul1 / aadA5 senB 1..149680 149680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T107929 WP_001159871.1 NZ_CP030770:c88125-87820 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107929 NZ_CP030770:c88125-87820 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT107929 WP_000813634.1 NZ_CP030770:c88345-88127 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107929 NZ_CP030770:c88345-88127 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References