Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 87820..88345 Replicon plasmid p2017C-4173W12
Accession NZ_CP030770
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag DSB71_RS28620 Protein ID WP_001159871.1
Coordinates 87820..88125 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag DSB71_RS28625 Protein ID WP_000813634.1
Coordinates 88127..88345 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS28585 83247..84044 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
DSB71_RS28590 84106..84357 + 252 Protein_106 DUF4113 domain-containing protein -
DSB71_RS28595 84362..84754 - 393 WP_000340835.1 plasmid partitioning/stability family protein -
DSB71_RS28600 84759..85730 - 972 WP_001103694.1 hypothetical protein -
DSB71_RS28605 85959..86603 + 645 WP_000633911.1 AAA family ATPase -
DSB71_RS28610 86597..86872 + 276 WP_000239529.1 hypothetical protein -
DSB71_RS28615 87010..87819 - 810 WP_000016979.1 site-specific integrase -
DSB71_RS28620 87820..88125 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
DSB71_RS28625 88127..88345 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DSB71_RS28635 90667..91308 + 642 WP_000990667.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / sul1 / aadA5 senB 1..149680 149680


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T107929 WP_001159871.1 NZ_CP030770:c88125-87820 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107929 NZ_CP030770:c88125-87820 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT107929 WP_000813634.1 NZ_CP030770:c88345-88127 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107929 NZ_CP030770:c88345-88127 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References