Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 12351..12844 Replicon plasmid pMCR-1_2017C-4173W12
Accession NZ_CP030769
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag DSB71_RS27885 Protein ID WP_001230709.1
Coordinates 12351..12605 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag DSB71_RS27890 Protein ID WP_000151456.1
Coordinates 12602..12844 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS27845 7924..8085 + 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -
DSB71_RS27850 8235..8831 + 597 WP_000125165.1 hypothetical protein -
DSB71_RS29535 8849..9133 - 285 WP_001162371.1 hypothetical protein -
DSB71_RS27860 9207..9872 - 666 WP_000532148.1 P-loop NTPase -
DSB71_RS27870 10460..10789 + 330 WP_000855119.1 hypothetical protein -
DSB71_RS27875 11193..11708 + 516 WP_001025393.1 J domain-containing protein -
DSB71_RS27880 11812..12354 + 543 WP_001083904.1 hypothetical protein -
DSB71_RS27885 12351..12605 + 255 WP_001230709.1 hypothetical protein Toxin
DSB71_RS27890 12602..12844 + 243 WP_000151456.1 hypothetical protein Antitoxin
DSB71_RS27900 13415..13708 + 294 WP_000673428.1 hypothetical protein -
DSB71_RS27905 14125..14307 - 183 WP_024135751.1 hypothetical protein -
DSB71_RS27910 14865..15047 + 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
DSB71_RS27915 15072..15509 + 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
DSB71_RS27920 15640..15966 - 327 WP_032345720.1 hypothetical protein -
DSB71_RS27925 15968..16408 - 441 WP_104652140.1 hypothetical protein -
DSB71_RS27930 16428..17135 - 708 WP_000108794.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..27128 27128


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(2-63)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T107928 WP_001230709.1 NZ_CP030769:12351-12605 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T107928 NZ_CP030769:12351-12605 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT107928 WP_000151456.1 NZ_CP030769:12602-12844 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT107928 NZ_CP030769:12602-12844 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References