Detailed information of TA system TA107915

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4404017..4404655
• Replicon: chromosome
• Accession: NZ_CP030768
• Organism: Escherichia coli strain 2017C-4173W12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: DSB71_RS23030
• Protein ID: WP_000813797.1
• Coordinates: 4404017..4404193 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: DSB71_RS23035
• Protein ID: WP_076611057.1
• Coordinates: 4404239..4404655 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB71_RS23010	4399639..4400850	-	1212	WP_001323162.1	BenE family transporter YdcO	-
DSB71_RS23015	4400903..4401439	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
DSB71_RS23020	4401512..4403473	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
DSB71_RS23025	4403565..4403795	-	231	WP_000494244.1	YncJ family protein	-
DSB71_RS23030	4404017..4404193	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
DSB71_RS23035	4404239..4404655	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
DSB71_RS23040	4404734..4406140	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
DSB71_RS23045	4406385..4407530	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
DSB71_RS23050	4407548..4408561	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
DSB71_RS23055	4408562..4409503	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T107915 WP_000813797.1 NZ_CP030768:4404017-4404193 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T107915 NZ_CP030768:4404017-4404193 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT107915 WP_076611057.1 NZ_CP030768:4404239-4404655 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT107915 NZ_CP030768:4404239-4404655 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715