Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3186113..3186731 Replicon chromosome
Accession NZ_CP030768
Organism Escherichia coli strain 2017C-4173W12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DSB71_RS16195 Protein ID WP_001291435.1
Coordinates 3186113..3186331 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DSB71_RS16200 Protein ID WP_000344800.1
Coordinates 3186357..3186731 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB71_RS16160 3181402..3181974 + 573 WP_000779826.1 lipoprotein -
DSB71_RS16165 3182005..3182316 - 312 WP_000409911.1 MGMT family protein -
DSB71_RS16175 3182695..3183048 + 354 WP_000878140.1 DUF1428 domain-containing protein -
DSB71_RS16180 3183090..3184640 - 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DSB71_RS16185 3184804..3185274 - 471 WP_000136192.1 YlaC family protein -
DSB71_RS16190 3185390..3185941 - 552 WP_000102559.1 maltose O-acetyltransferase -
DSB71_RS16195 3186113..3186331 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DSB71_RS16200 3186357..3186731 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DSB71_RS16205 3187277..3190426 - 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
DSB71_RS16210 3190449..3191642 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107908 WP_001291435.1 NZ_CP030768:c3186331-3186113 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T107908 NZ_CP030768:c3186331-3186113 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT107908 WP_000344800.1 NZ_CP030768:c3186731-3186357 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT107908 NZ_CP030768:c3186731-3186357 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References