Detailed information of TA system TA107894

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 992975..993809
• Replicon: chromosome
• Accession: NZ_CP030768
• Organism: Escherichia coli strain 2017C-4173W12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: DSB71_RS05010
• Protein ID: WP_000854689.1
• Coordinates: 993432..993809 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0IVP8
• Locus tag: DSB71_RS05005
• Protein ID: WP_001285599.1
• Coordinates: 992975..993355 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB71_RS04970	989062..989517	+	456	WP_001309747.1	hypothetical protein	-
DSB71_RS29485	989618..989749	+	132	WP_106108514.1	DUF905 family protein	-
DSB71_RS04980	989928..990749	+	822	WP_001234555.1	DUF945 domain-containing protein	-
DSB71_RS04985	991013..991486	+	474	WP_001538139.1	antirestriction protein	-
DSB71_RS04990	991502..991978	+	477	WP_001186761.1	RadC family protein	-
DSB71_RS04995	992041..992262	+	222	WP_000692315.1	DUF987 domain-containing protein	-
DSB71_RS05000	992281..992925	+	645	WP_000086757.1	hypothetical protein	-
DSB71_RS05005	992975..993355	+	381	WP_001285599.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
DSB71_RS05010	993432..993809	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
DSB71_RS05015	993806..994294	+	489	WP_000761698.1	hypothetical protein	-
DSB71_RS05020	994311..994508	+	198	WP_000839250.1	DUF957 domain-containing protein	-
DSB71_RS05025	994593..995438	+	846	WP_001446750.1	DUF4942 domain-containing protein	-
DSB71_RS05030	995507..995902	+	396	WP_000208381.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
DSB71_RS05035	995895..996828	+	934	Protein_921	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
DSB71_RS29075	997260..997415	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	997260..997415	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T107894 WP_000854689.1 NZ_CP030768:993432-993809 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T107894 NZ_CP030768:993432-993809 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13984.78 Da
• Isoelectric Point: 5.0823

>AT107894 WP_001285599.1 NZ_CP030768:992975-993355 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT107894 NZ_CP030768:992975-993355 [Escherichia coli]
ATGTCGGACACACTCCCCGGAACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGTTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGGGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGGTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG