Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3935777..3936408 Replicon chromosome
Accession NZ_CP030767
Organism Escherichia coli strain 2017C-4109

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag DSB73_RS21660 Protein ID WP_001259385.1
Coordinates 3936133..3936408 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag DSB73_RS21655 Protein ID WP_000593555.1
Coordinates 3935777..3936136 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB73_RS21625 3930889..3931362 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
DSB73_RS21630 3931359..3931640 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
DSB73_RS21635 3931717..3933075 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
DSB73_RS21640 3933068..3934576 + 1509 WP_001301773.1 carbohydrate kinase -
DSB73_RS21645 3934566..3934835 + 270 WP_000933060.1 HPr family phosphocarrier protein -
DSB73_RS21650 3934867..3935727 + 861 WP_001131788.1 class II aldolase -
DSB73_RS21655 3935777..3936136 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
DSB73_RS21660 3936133..3936408 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
DSB73_RS21665 3936481..3937605 - 1125 WP_001301659.1 ABC transporter permease -
DSB73_RS21670 3937605..3940340 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
DSB73_RS21675 3940337..3941404 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T107880 WP_001259385.1 NZ_CP030767:c3936408-3936133 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T107880 NZ_CP030767:c3936408-3936133 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT107880 WP_000593555.1 NZ_CP030767:c3936136-3935777 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT107880 NZ_CP030767:c3936136-3935777 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References