Detailed information of TA system TA107864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1709127..1709598
• Replicon: chromosome
• Accession: NZ_CP030767
• Organism: Escherichia coli strain 2017C-4109

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: DSB73_RS09705
• Protein ID: WP_001303511.1
• Coordinates: 1709320..1709598 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: DSB73_RS09700
• Protein ID: WP_001302048.1
• Coordinates: 1709127..1709318 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DSB73_RS09660	1704334..1704918	-	585	WP_000206793.1	DUF551 domain-containing protein	-
DSB73_RS09665	1704975..1705370	-	396	WP_001118159.1	DUF977 family protein	-
DSB73_RS09670	1705386..1706155	-	770	Protein_1634	DUF1627 domain-containing protein	-
DSB73_RS09675	1706181..1706921	-	741	WP_000788938.1	ATP-binding protein	-
DSB73_RS09680	1706928..1707890	-	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
DSB73_RS09685	1707913..1708338	-	426	WP_000693943.1	Rha family transcriptional regulator	-
DSB73_RS09690	1708335..1708637	-	303	WP_000172738.1	transcriptional regulator	-
DSB73_RS09695	1708735..1709106	+	372	WP_001169686.1	hypothetical protein	-
DSB73_RS09700	1709127..1709318	+	192	WP_001302048.1	hypothetical protein	Antitoxin
DSB73_RS09705	1709320..1709598	+	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DSB73_RS09715	1709950..1710249	+	300	WP_001240334.1	hypothetical protein	-
DSB73_RS09720	1710321..1710539	+	219	WP_001171930.1	hypothetical protein	-
DSB73_RS09730	1711108..1711296	+	189	WP_000449192.1	cell division inhibition protein DicB	-
DSB73_RS09735	1711293..1711484	+	192	WP_001090200.1	DUF1482 family protein	-
DSB73_RS09740	1711577..1714048	+	2472	WP_000048458.1	exonuclease	-
DSB73_RS09745	1714121..1714372	+	252	WP_000005552.1	excisionase family protein	-
DSB73_RS28840	1714407..1714591	+	185	Protein_1648	DUF3596 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1668325..1717884	49559

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T107864 WP_001303511.1 NZ_CP030767:1709320-1709598 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T107864 NZ_CP030767:1709320-1709598 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT107864 WP_001302048.1 NZ_CP030767:1709127-1709318 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT107864 NZ_CP030767:1709127-1709318 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0