107856

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 10089..10582 Replicon plasmid pMCR-1
Accession NZ_CP030766
Organism Escherichia coli strain 2017C-4109

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag DSB73_RS00640 Protein ID WP_001230709.1
Coordinates 10328..10582 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag DSB73_RS00635 Protein ID WP_000151456.1
Coordinates 10089..10331 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DSB73_RS00595 5798..6505 + 708 WP_000108794.1 hypothetical protein -
DSB73_RS00600 6525..6965 + 441 WP_104652140.1 hypothetical protein -
DSB73_RS00605 6967..7293 + 327 WP_032345720.1 hypothetical protein -
DSB73_RS00610 7424..7861 - 438 WP_000466318.1 type II toxin-antitoxin system HicB family antitoxin -
DSB73_RS00615 7886..8068 - 183 WP_000833470.1 type II toxin-antitoxin system HicA family toxin -
DSB73_RS00620 8626..8808 + 183 WP_024135751.1 hypothetical protein -
DSB73_RS00625 9225..9518 - 294 WP_000673428.1 hypothetical protein -
DSB73_RS00635 10089..10331 - 243 WP_000151456.1 hypothetical protein Antitoxin
DSB73_RS00640 10328..10582 - 255 WP_001230709.1 hypothetical protein Toxin
DSB73_RS00645 10579..11121 - 543 WP_001083904.1 hypothetical protein -
DSB73_RS00650 11225..11740 - 516 WP_001025393.1 J domain-containing protein -
DSB73_RS00655 12144..12473 - 330 WP_000855119.1 hypothetical protein -
DSB73_RS00665 13061..13726 + 666 WP_000532148.1 P-loop NTPase -
DSB73_RS29110 13800..14084 + 285 WP_001162371.1 hypothetical protein -
DSB73_RS00675 14102..14698 - 597 WP_000125165.1 hypothetical protein -
DSB73_RS00680 14848..15009 - 162 WP_000775236.1 type I toxin-antitoxin system Hok family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..59808 59808


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-133)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-36)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T107856 WP_001230709.1 NZ_CP030766:c10582-10328 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T107856 NZ_CP030766:c10582-10328 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT107856 WP_000151456.1 NZ_CP030766:c10331-10089 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT107856 NZ_CP030766:c10331-10089 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References