Detailed information of TA system TA107854

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 361314..361984
• Replicon: plasmid unnamed4
• Accession: NZ_CP030764
• Organism: Rhizobium leguminosarum strain ATCC 14479

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DLJ82_RS37900
• Protein ID: WP_018246604.1
• Coordinates: 361314..361718 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: DLJ82_RS37905
• Protein ID: WP_112908684.1
• Coordinates: 361715..361984 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ82_RS37870	356461..357279	+	819	WP_026188779.1	(S)-ureidoglycine aminohydrolase	-
DLJ82_RS37875	357299..357820	+	522	WP_018246596.1	GNAT family N-acetyltransferase	-
DLJ82_RS37880	357863..358216	+	354	WP_018517210.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DLJ82_RS37885	358231..358545	-	315	WP_011655201.1	helix-turn-helix domain-containing protein	-
DLJ82_RS37890	359061..359798	+	738	WP_026188781.1	hypothetical protein	-
DLJ82_RS39840	359805..359966	-	162	WP_018246599.1	hypothetical protein	-
DLJ82_RS37895	360092..361093	-	1002	WP_112903108.1	IS30 family transposase	-
DLJ82_RS37900	361314..361718	-	405	WP_018246604.1	type II toxin-antitoxin system VapC family toxin	Toxin
DLJ82_RS37905	361715..361984	-	270	WP_112908684.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
DLJ82_RS37910	362209..362427	-	219	WP_003547964.1	hypothetical protein	-
DLJ82_RS37915	362488..363333	-	846	WP_112908878.1	pirin family protein	-
DLJ82_RS37920	363419..365179	-	1761	WP_112908685.1	MOSC domain-containing protein	-
DLJ82_RS37925	365232..365789	-	558	WP_112908686.1	G/U mismatch-specific DNA glycosylase	-
DLJ82_RS37930	365939..366424	+	486	WP_112908687.1	DMT family transporter	-
DLJ82_RS37935	366421..366861	+	441	WP_112908688.1	DMT family transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..630306	630306
	-	flank	IS/Tn	-	-	360092..361093	1001

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-117)

Antitoxin

• SpoVT-AbrB domain (18-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14188.56 Da
• Isoelectric Point: 5.1421

>T107854 WP_018246604.1 NZ_CP030764:c361718-361314 [Rhizobium leguminosarum]
VSRLYMLDTNIVSELARNPMGTITRRIAEVGPDAICVSIITASELRYGCAKKGSPKLLAQIEAILGSIPVLALDLPADAE
YGGIRAELEGAGKPTGPNDLFIAAHASALGAVLVTANMGEFMRIRALQVENWLI

Nucleotide

• Length: 405 bp

>T107854 NZ_CP030764:c361718-361314 [Rhizobium leguminosarum]
GTGAGCAGGCTTTATATGCTGGACACCAATATCGTCTCGGAGCTTGCTCGAAACCCGATGGGTACAATCACCCGACGCAT
CGCCGAGGTTGGGCCCGATGCAATTTGCGTCAGCATTATCACGGCGTCGGAGTTGCGCTACGGGTGCGCCAAGAAGGGAT
CACCGAAACTTCTAGCACAGATCGAAGCCATCCTTGGCAGCATACCAGTGCTTGCGCTGGATCTGCCCGCCGATGCTGAA
TACGGTGGCATCCGGGCTGAACTGGAGGGTGCTGGCAAGCCTACCGGTCCAAATGACTTATTCATCGCTGCCCACGCCTC
TGCCCTCGGAGCAGTTCTGGTGACGGCGAACATGGGCGAGTTCATGCGTATCCGCGCTTTGCAGGTCGAAAACTGGTTGA
TTTGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10188.56 Da
• Isoelectric Point: 4.5610

>AT107854 WP_112908684.1 NZ_CP030764:c361984-361715 [Rhizobium leguminosarum]
VVNSRRQEPTPQREAKLFRNNKSQAVRIPADFELPGDRVMIHRDGDRLIIEPVHRKNLLEVLAGLEPLGPEDQFPDVDDT
LLPVEEIDL

Nucleotide

• Length: 270 bp

>AT107854 NZ_CP030764:c361984-361715 [Rhizobium leguminosarum]
GTGGTCAATTCCCGACGTCAAGAGCCTACTCCCCAGCGGGAGGCCAAGCTTTTCAGAAACAACAAGAGTCAGGCTGTACG
AATCCCGGCTGACTTTGAGCTACCCGGTGACAGAGTGATGATCCACCGCGACGGCGATCGGCTCATTATAGAGCCTGTGC
ACCGGAAGAACCTCCTGGAAGTTCTGGCTGGTCTGGAGCCGCTTGGTCCTGAAGATCAGTTTCCCGATGTCGACGATACC
CTGCTTCCTGTGGAGGAAATCGACCTGTGA