Detailed information of TA system TA107853

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacT-ataR/DUF1778(antitoxin)
• Location: 98270..99042
• Replicon: plasmid unnamed4
• Accession: NZ_CP030764
• Organism: Rhizobium leguminosarum strain ATCC 14479

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2Z4YVU8
• Locus tag: DLJ82_RS36640
• Protein ID: WP_018246328.1
• Coordinates: 98554..99042 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: DLJ82_RS36635
• Protein ID: WP_112908529.1
• Coordinates: 98270..98554 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ82_RS36615	93472..95880	+	2409	WP_112908526.1	ABC transporter permease	-
DLJ82_RS36620	95870..96937	+	1068	WP_112908527.1	iron ABC transporter permease	-
DLJ82_RS36625	97045..97794	+	750	WP_112908528.1	sulfite exporter TauE/SafE family protein	-
DLJ82_RS36635	98270..98554	+	285	WP_112908529.1	DUF1778 domain-containing protein	Antitoxin
DLJ82_RS36640	98554..99042	+	489	WP_018246328.1	GNAT family N-acetyltransferase	Toxin
DLJ82_RS36650	100055..100315	+	261	WP_017957861.1	DUF982 domain-containing protein	-
DLJ82_RS36655	100336..100539	-	204	Protein_111	nuclease	-
DLJ82_RS36660	100639..100929	-	291	WP_112908530.1	CopG family transcriptional regulator	-
DLJ82_RS36670	101362..101787	-	426	WP_112908531.1	hypothetical protein	-
DLJ82_RS36680	102242..102943	+	702	WP_112908533.1	DUF899 family protein	-
DLJ82_RS36685	102963..103592	-	630	WP_065276511.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..630306	630306

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (41-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-86)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17293.88 Da
• Isoelectric Point: 7.9271

>T107853 WP_018246328.1 NZ_CP030764:98554-99042 [Rhizobium leguminosarum]
MLSAPTLLGEAHDLQLFDSGHDSLDEWLRRRARANQVSGASRTYVVCEGERVVGYYCLSSGALAVSDAPGAIRRNMPDPV
PMAVLGRLAIDRNWQGKGIGAALLQDAVLRTGQAAHIMGIRGLLVHAISDEAKAFYEHFGFAASPANPMALVLSLKAQVG
LK

Nucleotide

• Length: 489 bp

>T107853 NZ_CP030764:98554-99042 [Rhizobium leguminosarum]
ATGTTGTCTGCACCGACGCTTCTCGGCGAAGCGCACGATCTCCAGCTATTCGACAGCGGGCACGACAGCCTTGATGAATG
GCTGCGCCGCAGGGCGCGGGCCAATCAGGTCAGCGGCGCATCGCGAACCTATGTGGTCTGCGAGGGCGAGCGCGTTGTCG
GTTATTACTGCCTCTCATCGGGAGCGCTGGCGGTCTCGGACGCTCCGGGAGCGATCCGGCGCAACATGCCCGACCCGGTT
CCCATGGCAGTCCTTGGCCGTCTGGCCATCGATCGCAACTGGCAGGGAAAAGGAATCGGAGCCGCGCTGTTGCAAGACGC
CGTGCTGCGTACCGGACAGGCAGCCCATATCATGGGTATTCGCGGCCTTCTGGTTCACGCGATCTCCGATGAGGCCAAGG
CCTTCTATGAGCATTTTGGATTTGCAGCGTCGCCCGCGAATCCTATGGCCTTGGTGTTATCATTGAAAGCTCAAGTAGGC
CTGAAGTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10683.06 Da
• Isoelectric Point: 8.6086

>AT107853 WP_112908529.1 NZ_CP030764:98270-98554 [Rhizobium leguminosarum]
MTSKNAPDTHTRAVNLRVREDVRVLIDRAAKAHGKTRSDFMIDASRRAAEDALLDQTLVRVDPDSYKHYLAVLDQPPNSE
GFERLMNAPKPWQP

Nucleotide

• Length: 285 bp

>AT107853 NZ_CP030764:98270-98554 [Rhizobium leguminosarum]
ATGACCAGCAAAAATGCCCCTGATACCCATACCCGCGCCGTTAACTTGAGGGTTCGTGAGGACGTTCGCGTCCTGATCGA
CCGCGCCGCCAAGGCCCACGGGAAAACCCGTTCGGATTTCATGATCGACGCATCGCGCCGTGCCGCAGAAGACGCGCTTC
TCGATCAGACATTGGTGCGGGTCGATCCTGACAGCTACAAACACTATCTCGCCGTCCTCGACCAGCCGCCAAATAGCGAA
GGCTTCGAACGGTTGATGAATGCTCCCAAACCCTGGCAGCCGTAA

Structures

Toxin

• AlphaFold DB: A0A2Z4YVU8