Detailed information of TA system TA107849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 1068919..1069412
• Replicon: plasmid unnamed1
• Accession: NZ_CP030761
• Organism: Rhizobium leguminosarum strain ATCC 14479

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: DLJ82_RS29380
• Protein ID: WP_112907697.1
• Coordinates: 1068919..1069173 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A559THZ3
• Locus tag: DLJ82_RS29385
• Protein ID: WP_028739446.1
• Coordinates: 1069170..1069412 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ82_RS29350	1063973..1064236	+	264	WP_112907693.1	WGR domain-containing protein	-
DLJ82_RS29355	1064255..1064878	-	624	WP_112907694.1	RES family NAD+ phosphorylase	-
DLJ82_RS29360	1064850..1065566	-	717	WP_112907695.1	hypothetical protein	-
DLJ82_RS29365	1065749..1066727	+	979	Protein_992	IS5 family transposase	-
DLJ82_RS29370	1066806..1067861	-	1056	WP_112907696.1	Fic family protein	-
DLJ82_RS29375	1068025..1068903	+	879	Protein_994	IS5 family transposase	-
DLJ82_RS29380	1068919..1069173	-	255	WP_112907697.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DLJ82_RS29385	1069170..1069412	-	243	WP_028739446.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
DLJ82_RS29390	1069607..1069699	-	93	Protein_997	IS5/IS1182 family transposase	-
DLJ82_RS29400	1070556..1071239	-	684	WP_112907699.1	IS6 family transposase	-
DLJ82_RS29410	1071616..1071723	+	108	Protein_999	DNA polymerase IV	-
DLJ82_RS29415	1071863..1072801	-	939	WP_112907700.1	phosphotransferase family protein	-
DLJ82_RS29420	1072813..1073781	-	969	WP_112907701.1	aminoglycoside phosphotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1234209	1234209
	-	inside	IScluster/Tn	-	-	1067983..1071239	3256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-74)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9625.09 Da
• Isoelectric Point: 6.2123

>T107849 WP_112907697.1 NZ_CP030761:c1069173-1068919 [Rhizobium leguminosarum]
MSFKLSAEAEEDIIAIAEQGVRMFGAGQAKRYHNELFALFDLIAVNPRIARERDEIDPPVRIHPFKAHLIVYQRWCGDQQ
ICGI

Nucleotide

• Length: 255 bp

>T107849 NZ_CP030761:c1069173-1068919 [Rhizobium leguminosarum]
ATGAGTTTTAAGCTTTCTGCCGAAGCGGAAGAGGACATTATTGCGATCGCCGAGCAAGGTGTCCGTATGTTCGGGGCAGG
CCAGGCAAAGCGATACCACAATGAACTTTTCGCATTATTTGATCTGATAGCTGTCAATCCACGCATCGCGCGCGAACGGG
ATGAGATTGATCCGCCCGTTAGAATTCATCCCTTCAAAGCGCATCTAATCGTTTATCAGCGTTGGTGCGGGGATCAGCAA
ATATGCGGAATTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8854.00 Da
• Isoelectric Point: 9.7765

>AT107849 WP_028739446.1 NZ_CP030761:c1069412-1069170 [Rhizobium leguminosarum]
MATMNVSLPGPMKDWVEAQARTGRYSNASDYVRDLIRRDQTRNDKIAAMQSFVEAGLQSGVGIRSKDELFAEAMARASKS

Nucleotide

• Length: 243 bp

>AT107849 NZ_CP030761:c1069412-1069170 [Rhizobium leguminosarum]
ATGGCCACGATGAACGTATCTCTTCCAGGCCCGATGAAGGATTGGGTGGAGGCCCAGGCCAGGACCGGGCGATATTCGAA
TGCTAGCGACTACGTGCGCGACCTGATCCGACGCGACCAGACGCGAAACGACAAGATCGCCGCCATGCAAAGCTTCGTCG
AGGCCGGGCTCCAAAGTGGTGTCGGCATCCGATCAAAGGATGAACTCTTTGCTGAAGCGATGGCTCGTGCCAGCAAGTCA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A559THZ3