Detailed information of TA system TA107848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 951690..952270
• Replicon: plasmid unnamed1
• Accession: NZ_CP030761
• Organism: Rhizobium leguminosarum strain ATCC 14479

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A444I7Z5
• Locus tag: DLJ82_RS28785
• Protein ID: WP_018245324.1
• Coordinates: 951887..952270 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7Y3WNA0
• Locus tag: DLJ82_RS28780
• Protein ID: WP_018245323.1
• Coordinates: 951690..951890 (+)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ82_RS28740	946720..948300	+	1581	WP_112907634.1	ABC transporter substrate-binding protein	-
DLJ82_RS28745	948372..948746	-	375	Protein_878	DUF5615 family PIN-like protein	-
DLJ82_RS28750	948743..949375	-	633	WP_112907635.1	DUF433 domain-containing protein	-
DLJ82_RS28755	949431..949562	+	132	Protein_880	transposase domain-containing protein	-
DLJ82_RS28760	949710..950294	-	585	Protein_881	glycerol-3-phosphate dehydrogenase	-
DLJ82_RS28765	950281..950625	+	345	WP_112907636.1	hypothetical protein	-
DLJ82_RS28770	950791..951204	+	414	WP_112907637.1	curlin	-
DLJ82_RS39345	951285..951524	+	240	WP_162710346.1	hypothetical protein	-
DLJ82_RS28780	951690..951890	+	201	WP_018245323.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DLJ82_RS28785	951887..952270	+	384	WP_018245324.1	type II toxin-antitoxin system VapC family toxin	Toxin
DLJ82_RS28790	952480..952707	+	228	WP_112907822.1	CopG family transcriptional regulator	-
DLJ82_RS28800	953274..953697	-	424	Protein_888	HlyD family secretion protein	-
DLJ82_RS39740	953928..954104	+	177	WP_018071841.1	hypothetical protein	-
DLJ82_RS28805	954139..955191	+	1053	WP_065275913.1	ribosome small subunit-dependent GTPase A	-
DLJ82_RS28810	955488..956582	-	1095	WP_018245327.1	hypothetical protein	-
DLJ82_RS28820	956998..957218	+	221	Protein_892	Gfo/Idh/MocA family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1234209	1234209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14117.31 Da
• Isoelectric Point: 9.1808

>T107848 WP_018245324.1 NZ_CP030761:951887-952270 [Rhizobium leguminosarum]
VILADTSIWIDHFRHVDAELRTIIEDDRLLCHPAVVGELALGSLRDRGRVIAFLAAQRQAFVATHDEVMTMIDRHGIFSM
GIGYTDAHLLASILLDRRAALWTRDKRLQAAAKKAGASLHKPANARN

Nucleotide

• Length: 384 bp

>T107848 NZ_CP030761:951887-952270 [Rhizobium leguminosarum]
GTGATACTGGCAGACACTTCTATATGGATCGATCATTTCCGCCATGTTGATGCGGAACTTCGGACGATCATTGAGGACGA
TCGTCTACTCTGCCATCCGGCTGTAGTTGGCGAACTGGCGCTTGGCAGCCTTCGAGATCGCGGGCGCGTGATAGCGTTTC
TGGCAGCCCAGCGCCAAGCGTTCGTCGCAACGCACGACGAAGTGATGACAATGATTGATCGTCACGGCATTTTCAGTATG
GGCATCGGCTATACAGATGCCCACTTGCTCGCCTCGATACTGCTTGATCGGCGAGCGGCGTTATGGACCAGGGACAAGCG
TCTTCAGGCGGCGGCCAAAAAGGCAGGCGCTTCATTGCATAAGCCGGCCAATGCTCGCAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7124.23 Da
• Isoelectric Point: 11.3760

>AT107848 WP_018245323.1 NZ_CP030761:951690-951890 [Rhizobium leguminosarum]
MRSTINLDDTLMERARSLTGTKETAAIVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT107848 NZ_CP030761:951690-951890 [Rhizobium leguminosarum]
ATGCGATCGACTATCAATCTAGACGACACTCTCATGGAAAGGGCCAGGTCCCTGACCGGCACAAAAGAAACCGCTGCTAT
AGTCCGCCAGGCGTTGGAGACGCTTGTCCGAGTAGAGTCCGGAAAACGCCTCATCGCGCTCGGCGGAACTATGCCCGATG
CAGAGGCAGCTCCACGTCGCCGGAGCGCAGCTGCCAAGTGA

Structures

Toxin

• AlphaFold DB: A0A444I7Z5

Antitoxin

• AlphaFold DB: A0A7Y3WNA0