Detailed information of TA system TA107847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 236520..237211
• Replicon: plasmid unnamed1
• Accession: NZ_CP030761
• Organism: Rhizobium leguminosarum strain ATCC 14479

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: DLJ82_RS25305
• Protein ID: WP_112906949.1
• Coordinates: 236783..237211 (+)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R0AD13
• Locus tag: DLJ82_RS25300
• Protein ID: WP_017991284.1
• Coordinates: 236520..236786 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ82_RS25265	231860..232117	+	258	WP_008536431.1	hypothetical protein	-
DLJ82_RS25270	232117..232488	+	372	WP_018245706.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DLJ82_RS25275	232638..233363	-	726	WP_112906945.1	amino acid ABC transporter ATP-binding protein	-
DLJ82_RS25280	233341..234000	-	660	WP_018245708.1	amino acid ABC transporter permease	-
DLJ82_RS25285	233997..234668	-	672	WP_112906947.1	amino acid ABC transporter permease	-
DLJ82_RS25290	234725..235510	-	786	WP_018245710.1	transporter substrate-binding domain-containing protein	-
DLJ82_RS25295	235514..236263	-	750	WP_018245711.1	GntR family transcriptional regulator	-
DLJ82_RS25300	236520..236786	+	267	WP_017991284.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
DLJ82_RS25305	236783..237211	+	429	WP_112906949.1	type II toxin-antitoxin system VapC family toxin	Toxin
DLJ82_RS25315	237307..238077	-	771	WP_112906953.1	amino acid ABC transporter ATP-binding protein	-
DLJ82_RS25320	238090..238749	-	660	WP_011648868.1	amino acid ABC transporter permease	-
DLJ82_RS25325	238756..239418	-	663	WP_047622998.1	amino acid ABC transporter permease	-
DLJ82_RS25330	239586..240425	-	840	WP_112906955.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1234209	1234209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (9-66)

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15915.12 Da
• Isoelectric Point: 4.4838

>T107847 WP_112906949.1 NZ_CP030761:236783-237211 [Rhizobium leguminosarum]
VRLLLDTNVLSEVTRPRPDAHVLQWLDSLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVAIAWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIEDFEGFEIELVDPWTERP

Nucleotide

• Length: 429 bp

>T107847 NZ_CP030761:236783-237211 [Rhizobium leguminosarum]
GTGAGACTTCTGCTGGACACGAATGTGCTTTCCGAGGTGACAAGGCCGCGTCCCGATGCACATGTCCTACAATGGCTGGA
CAGTCTCGACGAGGATCGCTCATTCATCAGCGTCGTATCGATCGCGGAGATCCGGCGTGGTGTTGCATTGATGGAAAGCG
GCCGGAAGCGCGACGCGCTGGCTGAATGGCTTGCCCAGGACCTGCCGCAGCGCTTCGAGCAGCGTGTCATCCCTGTGGAT
CAGCCGGTTGCGATAGCTTGGGGCGACCTGATGGGGCTCGCAAAACGAAGCGGGCGCGGCTTGTCGTCAATGGACGGCCT
GATTGCCGCCACCGCGATTGCTCACGATCTCACCCTCGCCACCCGTAATATAGAGGATTTCGAGGGCTTTGAGATCGAGC
TTGTCGACCCTTGGACCGAGCGCCCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9960.34 Da
• Isoelectric Point: 10.0842

>AT107847 WP_017991284.1 NZ_CP030761:236520-236786 [Rhizobium leguminosarum]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL

Nucleotide

• Length: 267 bp

>AT107847 NZ_CP030761:236520-236786 [Rhizobium leguminosarum]
ATGCAGCCATCACGACCAGAGGATAAAAGTTGGACCGTCGCCAGTGCGAAGGCCAAGCTGTCTGAGGTTATCGAACGCGC
GCAAGCAACGCCGCAAACAATCATGCGCAATGGCAAACCAAGCGTCGTCGTCGTTTCGGCCGAGGAATGGCAGCGGAAGA
CCGCGCGCAAGGGCACCCTTGCAGAGTTTCTGATGCAATCGCCGCTGCGTGGCGCCGATCTGGACCTCGAACGGCAGCGC
GACGAACCGCGTGATCTTCAGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4R0AD13