Detailed information of TA system TA107846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 117207..118009
• Replicon: plasmid unnamed1
• Accession: NZ_CP030761
• Organism: Rhizobium leguminosarum strain ATCC 14479

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: DLJ82_RS24765
• Protein ID: WP_112906854.1
• Coordinates: 117482..118009 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: A0A7Y3SJF5
• Locus tag: DLJ82_RS24760
• Protein ID: WP_018245607.1
• Coordinates: 117207..117485 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DLJ82_RS24745	113109..113588	+	480	WP_204361389.1	MEKHLA domain-containing protein	-
DLJ82_RS24750	113736..115850	+	2115	WP_112906852.1	carboxy terminal-processing peptidase	-
DLJ82_RS24755	116174..117052	+	879	WP_112906853.1	exopolysaccharide synthesis protein	-
DLJ82_RS24760	117207..117485	+	279	WP_018245607.1	DUF1778 domain-containing protein	Antitoxin
DLJ82_RS24765	117482..118009	+	528	WP_112906854.1	GNAT family N-acetyltransferase	Toxin
DLJ82_RS24770	118292..119938	+	1647	WP_112906855.1	acetolactate synthase large subunit	-
DLJ82_RS24775	119940..121331	+	1392	WP_112906856.1	aldehyde dehydrogenase family protein	-
DLJ82_RS24780	121450..122529	+	1080	WP_112906857.1	nitronate monooxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1234209	1234209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18886.86 Da
• Isoelectric Point: 9.1665

>T107846 WP_112906854.1 NZ_CP030761:117482-118009 [Rhizobium leguminosarum]
VTLPVWHEEPIAKSHDRASFDCGDAAMNEFIRRFARQSHEQNAAKTFCAIDKAHPDRILGFYTVAPSAVTHEAVPPKMTR
GLARHEVAGFKLARLATDIKVAGKGLGGQLIAAAALRCLRLASEGGGILLIIDAKSERAAHWYASYGAEPLQEKPLTLVM
PLATFAADFKAKGLL

Nucleotide

• Length: 528 bp

>T107846 NZ_CP030761:117482-118009 [Rhizobium leguminosarum]
GTGACCCTCCCCGTCTGGCATGAAGAGCCGATCGCTAAGTCGCACGATCGGGCCTCATTCGATTGCGGTGATGCGGCAAT
GAACGAGTTCATTCGGCGCTTCGCCCGGCAGAGCCATGAGCAGAATGCGGCGAAGACCTTCTGCGCTATCGACAAGGCCC
ACCCTGATCGCATCCTCGGATTCTACACAGTCGCGCCCTCTGCCGTTACGCATGAGGCCGTGCCTCCGAAGATGACGAGA
GGCCTCGCACGCCATGAAGTCGCAGGCTTCAAGCTCGCGCGCTTGGCAACCGACATAAAGGTGGCGGGAAAAGGTTTAGG
CGGTCAGCTCATCGCTGCTGCAGCGCTCCGTTGCCTGCGGCTCGCCAGCGAGGGCGGCGGGATCCTGCTAATCATCGATG
CCAAGAGCGAGCGCGCCGCCCACTGGTATGCCAGCTACGGCGCCGAACCGCTGCAAGAGAAACCGCTCACCCTCGTTATG
CCGCTTGCCACCTTCGCCGCCGACTTCAAGGCCAAGGGGCTCCTGTAG

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10222.91 Da
• Isoelectric Point: 9.8602

>AT107846 WP_018245607.1 NZ_CP030761:117207-117485 [Rhizobium leguminosarum]
MPRTADNKSERYQLRIPPKQKAMIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLERPPKPNAR
LRAAISALPDAL

Nucleotide

• Length: 279 bp

>AT107846 NZ_CP030761:117207-117485 [Rhizobium leguminosarum]
ATGCCGAGAACCGCTGACAATAAATCCGAACGCTACCAGCTGCGCATCCCGCCAAAGCAGAAGGCAATGATCGCGCGCGC
AGCTGCGCTTTCGAACAAGGATATGGCCGATTTCATTCTCGATAAGATAGTACCTGAAGCCGAAGCGGTCATCCAGGCTG
CTGAAGTTGAGACCGTTTCGAGCCGCGATTTCATCCGCATCCTCGACCTGCTTGAACGCCCGCCCAAACCGAACGCGAGA
CTACGCGCCGCGATCTCTGCTCTACCGGATGCCTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Y3SJF5