Detailed information of TA system TA107835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1502540..1503048
• Replicon: chromosome
• Accession: NZ_CP030753
• Organism: Actinobacillus pleuropneumoniae strain S4074

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B0BQK9
• Locus tag: DRF63_RS06950
• Protein ID: WP_005605064.1
• Coordinates: 1502788..1503048 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B0BQK8
• Locus tag: DRF63_RS06945
• Protein ID: WP_005598318.1
• Coordinates: 1502540..1502791 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRF63_RS06905	1498077..1498625	-	549	WP_005598298.1	type IV pilus biogenesis/stability protein PilW	-
DRF63_RS06910	1498679..1499860	-	1182	WP_005598300.1	bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN	-
DRF63_RS06935	1500764..1502203	+	1440	WP_005598316.1	glutamate--tRNA ligase	-
DRF63_RS06945	1502540..1502791	+	252	WP_005598318.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
DRF63_RS06950	1502788..1503048	+	261	WP_005605064.1	Txe/YoeB family addiction module toxin	Toxin
DRF63_RS06955	1503207..1503374	-	168	WP_005598320.1	Trm112 family protein	-
DRF63_RS06960	1503376..1504356	-	981	WP_005598322.1	tetraacyldisaccharide 4'-kinase	-
DRF63_RS06965	1504450..1505709	-	1260	WP_005598324.1	ATP-dependent protease ATP-binding subunit ClpX	-
DRF63_RS06970	1505709..1506299	-	591	WP_005598326.1	ATP-dependent Clp endopeptidase proteolytic subunit ClpP	-
DRF63_RS06975	1506421..1506813	+	393	WP_005620423.1	SufE family protein	-
DRF63_RS06990	1507172..1507945	-	774	WP_005598329.1	DNA polymerase III subunit epsilon	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10423.99 Da
• Isoelectric Point: 8.0109

>T107835 WP_005605064.1 NZ_CP030753:1502788-1503048 [Actinobacillus pleuropneumoniae]
MKLTFSSNAWEDYLYWQKTDKIILKRINSLIKDIQRQPFEGIGKLEPLKFNLSGFWSRRINEEHRLIYSVEDEAILIVAC
RYHYDQ

Nucleotide

• Length: 261 bp

>T107835 NZ_CP030753:1502788-1503048 [Actinobacillus pleuropneumoniae]
ATGAAGCTAACTTTCTCGTCTAATGCGTGGGAAGATTATTTATACTGGCAAAAAACCGATAAAATAATTTTGAAACGTAT
AAACAGCCTGATCAAAGATATTCAACGCCAACCATTCGAAGGCATAGGAAAACTGGAGCCGCTAAAATTCAATCTTTCGG
GATTTTGGTCAAGGCGAATCAATGAAGAACACCGCTTAATTTACAGCGTGGAAGATGAAGCAATTTTAATAGTAGCGTGT
AGATACCATTATGATCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9362.66 Da
• Isoelectric Point: 4.5659

>AT107835 WP_005598318.1 NZ_CP030753:1502540-1502791 [Actinobacillus pleuropneumoniae]
MQAITYTEARQNLAGTMSKVAEDFEPILITRSKGGNCVLMSYEQYCSLEETAYLMRSPTNAKRLLDSVAELRQGQGIERE
LIE

Nucleotide

• Length: 252 bp

>AT107835 NZ_CP030753:1502540-1502791 [Actinobacillus pleuropneumoniae]
ATGCAAGCAATAACCTACACAGAAGCACGCCAAAATCTAGCCGGTACGATGTCAAAAGTAGCAGAAGATTTTGAGCCTAT
TTTAATTACAAGAAGCAAGGGCGGAAATTGCGTACTTATGTCGTATGAGCAGTATTGTTCACTTGAAGAAACCGCCTATT
TGATGCGTTCACCCACCAATGCAAAACGGCTATTGGATAGTGTGGCGGAATTAAGACAGGGACAAGGCATTGAAAGAGAG
CTAATCGAATGA

Structures

Toxin

• AlphaFold DB: B0BQK9

Antitoxin

• AlphaFold DB: B0BQK8