107831

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 833984..834508 Replicon chromosome
Accession NZ_CP030715
Organism Staphylococcus pseudintermedius strain VB88

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag DNH96_RS04210 Protein ID WP_014613379.1
Coordinates 833984..834337 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag DNH96_RS04215 Protein ID WP_014613378.1
Coordinates 834338..834508 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNH96_RS04190 (DNH96_04170) 831293..832063 - 771 WP_015729428.1 RNA polymerase sigma factor SigB -
DNH96_RS04195 (DNH96_04175) 832038..832517 - 480 WP_031253870.1 anti-sigma B factor RsbW -
DNH96_RS04200 (DNH96_04180) 832517..832843 - 327 WP_014613381.1 anti-sigma factor antagonist -
DNH96_RS04205 (DNH96_04185) 832922..833926 - 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
DNH96_RS04210 (DNH96_04190) 833984..834337 - 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DNH96_RS04215 (DNH96_04195) 834338..834508 - 171 WP_014613378.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DNH96_RS04220 (DNH96_04200) 834593..835741 - 1149 WP_014613377.1 alanine racemase -
DNH96_RS04225 (DNH96_04205) 835775..836125 - 351 WP_014613376.1 holo-ACP synthase -
DNH96_RS04230 (DNH96_04210) 836122..836640 - 519 WP_065354119.1 PH domain-containing protein -
DNH96_RS04235 (DNH96_04215) 836624..838153 - 1530 WP_189908859.1 PH domain-containing protein -
DNH96_RS04240 (DNH96_04220) 838146..838619 - 474 WP_014613373.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T107831 WP_014613379.1 NZ_CP030715:c834337-833984 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T107831 NZ_CP030715:c834337-833984 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATTCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT107831 WP_014613378.1 NZ_CP030715:c834508-834338 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT107831 NZ_CP030715:c834508-834338 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References