Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2397657..2398181 Replicon chromosome
Accession NZ_CP030374
Organism Staphylococcus pseudintermedius strain AH18

Toxin (Protein)


Gene name mazF Uniprot ID A0A2A4EDC7
Locus tag DNH82_RS11230 Protein ID WP_014613379.1
Coordinates 2397657..2398010 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A166MM16
Locus tag DNH82_RS11235 Protein ID WP_014613378.1
Coordinates 2398011..2398181 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DNH82_RS11210 (DNH82_11320) 2394966..2395736 - 771 WP_015729428.1 RNA polymerase sigma factor SigB -
DNH82_RS11215 (DNH82_11325) 2395711..2396190 - 480 WP_031253870.1 anti-sigma B factor RsbW -
DNH82_RS11220 (DNH82_11330) 2396190..2396516 - 327 WP_014613381.1 anti-sigma factor antagonist -
DNH82_RS11225 (DNH82_11335) 2396595..2397599 - 1005 WP_014613380.1 PP2C family protein-serine/threonine phosphatase -
DNH82_RS11230 (DNH82_11340) 2397657..2398010 - 354 WP_014613379.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DNH82_RS11235 (DNH82_11345) 2398011..2398181 - 171 WP_014613378.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
DNH82_RS11240 (DNH82_11350) 2398266..2399414 - 1149 WP_014613377.1 alanine racemase -
DNH82_RS11245 (DNH82_11355) 2399448..2399798 - 351 WP_014613376.1 holo-ACP synthase -
DNH82_RS11250 (DNH82_11360) 2399795..2400313 - 519 WP_014613375.1 PH domain-containing protein -
DNH82_RS11255 (DNH82_11365) 2400297..2401826 - 1530 WP_096533708.1 PH domain-containing protein -
DNH82_RS11260 (DNH82_11370) 2401819..2402292 - 474 WP_014613373.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-108)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13130.36 Da        Isoelectric Point: 10.4439

>T107829 WP_014613379.1 NZ_CP030374:c2398010-2397657 [Staphylococcus pseudintermedius]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKNKYKLDKDSVILLEQIRT
VDKKRLKEKLTFLSEKKMKEVNNAIGISLGLHMNHHK

Download         Length: 354 bp

>T107829 NZ_CP030374:c2398010-2397657 [Staphylococcus pseudintermedius]
ATGAAGCGCGGAGACGTTTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTTGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAACAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6396.04 Da        Isoelectric Point: 3.9930

>AT107829 WP_014613378.1 NZ_CP030374:c2398181-2398011 [Staphylococcus pseudintermedius]
MSAFNQTRFKNLEQSLKEGYVQMADLNLSLATEAYPVECEACDCNESHLTTDYKED

Download         Length: 171 bp

>AT107829 NZ_CP030374:c2398181-2398011 [Staphylococcus pseudintermedius]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTACA
AGGAAGACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A4EDC7

Antitoxin

Source ID Structure
AlphaFold DB A0A166MM16

References