Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2294523..2295144 Replicon chromosome
Accession NZ_CP030352
Organism Novosphingobium sp. P6W

Toxin (Protein)


Gene name vapC Uniprot ID A0A2Z5EBC7
Locus tag TQ38_RS11135 Protein ID WP_043972577.1
Coordinates 2294779..2295144 (+) Length 122 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A2Z5EBN3
Locus tag TQ38_RS11130 Protein ID WP_043972579.1
Coordinates 2294523..2294756 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TQ38_RS11110 2290264..2291142 - 879 WP_043972588.1 isocitrate lyase/PEP mutase family protein -
TQ38_RS11115 2291234..2291929 - 696 WP_043972585.1 HAD-IA family hydrolase -
TQ38_RS11120 2291989..2293380 + 1392 WP_043972583.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
TQ38_RS11125 2293429..2294172 - 744 WP_043972581.1 diguanylate cyclase -
TQ38_RS11130 2294523..2294756 + 234 WP_043972579.1 CopG family transcriptional regulator Antitoxin
TQ38_RS11135 2294779..2295144 + 366 WP_043972577.1 type II toxin-antitoxin system VapC family toxin Toxin
TQ38_RS11140 2295162..2296985 + 1824 WP_043972574.1 glutamine--fructose-6-phosphate transaminase (isomerizing) -
TQ38_RS11145 2297161..2299518 + 2358 WP_043972571.1 bifunctional diguanylate cyclase/phosphodiesterase -
TQ38_RS30575 2299595..2299771 + 177 WP_205316026.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-106)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 122 a.a.        Molecular weight: 13665.76 Da        Isoelectric Point: 8.5367

>T107827 WP_043972577.1 NZ_CP030352:2294779-2295144 [Novosphingobium sp. P6W]
VADPFFDTNILIDWLKDRPQAAAELSRYDRHRISRIVWTEILAGEPPETRPRVQQLISYFEVVEIEARAATAAADIRNRT
KIKLLDALVLATAQVHGAILVTRNTKDFPASMPGVRIPYTL

Download         Length: 366 bp

>T107827 NZ_CP030352:2294779-2295144 [Novosphingobium sp. P6W]
GTGGCCGATCCCTTCTTCGACACCAACATCCTGATCGACTGGCTGAAGGATCGTCCGCAGGCCGCGGCGGAACTGTCGCG
CTATGACCGTCACCGGATCAGCCGAATCGTTTGGACCGAAATCCTGGCCGGTGAACCGCCGGAAACGCGGCCCAGAGTGC
AGCAGCTGATCTCTTACTTCGAGGTCGTCGAAATCGAGGCCCGCGCTGCCACGGCGGCCGCGGACATCCGAAACCGCACG
AAGATCAAATTGCTCGACGCCCTGGTCCTGGCAACCGCCCAGGTGCACGGCGCCATTCTCGTTACACGAAATACCAAGGA
TTTTCCGGCAAGCATGCCGGGCGTCCGCATCCCCTATACCCTCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8888.79 Da        Isoelectric Point: 5.6902

>AT107827 WP_043972579.1 NZ_CP030352:2294523-2294756 [Novosphingobium sp. P6W]
MTRILADLPDEDIRWLDDRAAQQGKSRASVLREAVSVYRAESSQDWIARGAGYWKHRDDIGDGVEHQRAMRADRTFD

Download         Length: 234 bp

>AT107827 NZ_CP030352:2294523-2294756 [Novosphingobium sp. P6W]
ATGACTCGGATCCTTGCAGACCTTCCCGATGAGGACATCCGCTGGCTCGATGACCGCGCGGCCCAGCAGGGCAAGTCGCG
CGCGTCGGTCCTGCGTGAGGCGGTCAGTGTCTACCGCGCCGAATCATCGCAGGACTGGATTGCGCGCGGGGCCGGCTACT
GGAAGCACCGCGACGATATCGGCGACGGCGTGGAACACCAGCGTGCCATGCGCGCCGACCGTACTTTCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z5EBC7

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z5EBN3

References