Detailed information of TA system TA107802

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 49389..49914
• Replicon: plasmid unnamed1
• Accession: NZ_CP030345
• Organism: Enterobacter hormaechei strain AR_038

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A9E1GGX2
• Locus tag: CSB67_RS00290
• Protein ID: WP_016946795.1
• Coordinates: 49389..49694 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: CSB67_RS00295
• Protein ID: WP_004197642.1
• Coordinates: 49696..49914 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSB67_RS00260	45195..45464	+	270	WP_111978107.1	hypothetical protein	-
CSB67_RS00270	45820..46686	-	867	WP_004118283.1	replication initiation protein	-
CSB67_RS26850	47221..47325	+	105	WP_032409716.1	hypothetical protein	-
CSB67_RS00275	47454..47711	+	258	WP_000764642.1	hypothetical protein	-
CSB67_RS00280	47769..48545	-	777	WP_049245386.1	site-specific integrase	-
CSB67_RS00285	48548..49231	-	684	WP_023280892.1	hypothetical protein	-
CSB67_RS00290	49389..49694	-	306	WP_016946795.1	type II toxin-antitoxin system toxin CcdB	Toxin
CSB67_RS00295	49696..49914	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
CSB67_RS00305	50483..51064	+	582	WP_009654315.1	hypothetical protein	-
CSB67_RS00310	51220..52221	+	1002	WP_032635019.1	Abi family protein	-
CSB67_RS00315	52360..52938	-	579	WP_032635068.1	hypothetical protein	-
CSB67_RS00320	53228..53476	+	249	WP_004200907.1	hypothetical protein	-
CSB67_RS00325	53473..54705	+	1233	WP_032249456.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / armA / sul1 / qacE / blaNDM-1 / aadA2 / dfrA12 / aac(3)-IId / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / aac(6')-Ib / ant(3'')-Ia / blaOXA-9	-	1..152715	152715

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11548.22 Da
• Isoelectric Point: 5.6831

>T107802 WP_016946795.1 NZ_CP030345:c49694-49389 [Enterobacter hormaechei]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T107802 NZ_CP030345:c49694-49389 [Enterobacter hormaechei]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT107802 WP_004197642.1 NZ_CP030345:c49914-49696 [Enterobacter hormaechei]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT107802 NZ_CP030345:c49914-49696 [Enterobacter hormaechei]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA