Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 50643..51286 Replicon plasmid unnamed2
Accession NZ_CP030342
Organism Klebsiella pneumoniae strain AR_362

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CSB99_RS27725 Protein ID WP_001044770.1
Coordinates 50870..51286 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CSB99_RS27720 Protein ID WP_001261282.1
Coordinates 50643..50873 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSB99_RS27675 45841..46314 + 474 WP_004152341.1 YkgJ family cysteine cluster protein -
CSB99_RS27680 46406..46636 + 231 WP_011977773.1 hypothetical protein -
CSB99_RS27685 47528..48310 - 783 WP_004152340.1 site-specific integrase -
CSB99_RS27690 48310..48642 - 333 WP_004152339.1 hypothetical protein -
CSB99_RS27695 48649..49047 - 399 WP_004171440.1 hypothetical protein -
CSB99_RS27700 49073..49402 - 330 WP_004152337.1 hypothetical protein -
CSB99_RS27705 49430..49738 - 309 WP_004152336.1 hypothetical protein -
CSB99_RS27715 50225..50686 - 462 WP_072093212.1 hypothetical protein -
CSB99_RS27720 50643..50873 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CSB99_RS27725 50870..51286 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CSB99_RS27730 51360..52070 + 711 WP_004152334.1 ATP-binding protein -
CSB99_RS27735 52104..52801 + 698 Protein_69 IS1 family transposase -
CSB99_RS27740 52817..52927 + 111 Protein_70 mercuric transport protein periplasmic component -
CSB99_RS27745 52963..53385 + 423 WP_001340589.1 organomercurial transporter MerC -
CSB99_RS27750 53437..55131 + 1695 WP_000105636.1 mercury(II) reductase -
CSB99_RS27755 55149..55511 + 363 WP_001277456.1 mercury resistance co-regulator MerD -
CSB99_RS27760 55508..55744 + 237 WP_000993386.1 broad-spectrum mercury transporter MerE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1A / blaOXA-9 / blaKPC-2 - 1..113639 113639
- flank IS/Tn - - 44453..45721 1268


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T107799 WP_001044770.1 NZ_CP030342:50870-51286 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T107799 NZ_CP030342:50870-51286 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT107799 WP_001261282.1 NZ_CP030342:50643-50873 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT107799 NZ_CP030342:50643-50873 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References