Detailed information of TA system TA107735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2456357..2456995
• Replicon: chromosome
• Accession: NZ_CP030331
• Organism: Escherichia coli strain AR_452

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: CSC39_RS13570
• Protein ID: WP_000813794.1
• Coordinates: 2456819..2456995 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: CSC39_RS13565
• Protein ID: WP_001270285.1
• Coordinates: 2456357..2456773 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC39_RS13545	2451509..2452450	-	942	WP_001251313.1	ABC transporter permease	-
CSC39_RS13550	2452451..2453464	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
CSC39_RS13555	2453482..2454627	-	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
CSC39_RS13560	2454872..2456278	-	1407	WP_000760590.1	PLP-dependent aminotransferase family protein	-
CSC39_RS13565	2456357..2456773	-	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
CSC39_RS13570	2456819..2456995	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
CSC39_RS13575	2457217..2457447	+	231	WP_000494244.1	YncJ family protein	-
CSC39_RS13580	2457539..2459500	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
CSC39_RS13585	2459573..2460109	-	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
CSC39_RS13590	2460162..2461376	+	1215	WP_001326689.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2461416..2462564	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T107735 WP_000813794.1 NZ_CP030331:c2456995-2456819 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T107735 NZ_CP030331:c2456995-2456819 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT107735 WP_001270285.1 NZ_CP030331:c2456773-2456357 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT107735 NZ_CP030331:c2456773-2456357 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB