Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1292275..1292900 Replicon chromosome
Accession NZ_CP030331
Organism Escherichia coli strain AR_452

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag CSC39_RS07610 Protein ID WP_000911329.1
Coordinates 1292502..1292900 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag CSC39_RS07605 Protein ID WP_000450524.1
Coordinates 1292275..1292502 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC39_RS07580 1288077..1288547 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
CSC39_RS07585 1288547..1289119 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
CSC39_RS07590 1289265..1290143 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
CSC39_RS07595 1290160..1291194 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
CSC39_RS07600 1291407..1292120 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
CSC39_RS07605 1292275..1292502 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
CSC39_RS07610 1292502..1292900 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
CSC39_RS07615 1293047..1293910 + 864 WP_001267498.1 neutral zinc metallopeptidase -
CSC39_RS07620 1293925..1295940 + 2016 WP_000829295.1 tRNA cytosine(34) acetyltransferase TmcA -
CSC39_RS07625 1296014..1296712 + 699 WP_000679823.1 esterase -
CSC39_RS07630 1296822..1297022 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T107724 WP_000911329.1 NZ_CP030331:1292502-1292900 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T107724 NZ_CP030331:1292502-1292900 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT107724 WP_000450524.1 NZ_CP030331:1292275-1292502 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT107724 NZ_CP030331:1292275-1292502 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References