Detailed information of TA system TA107714

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 31515..32109
• Replicon: plasmid unnamed1
• Accession: NZ_CP030329
• Organism: Escherichia coli strain AR_452

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CSC39_RS00235
• Protein ID: WP_001403920.1
• Coordinates: 31515..31790 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: G3CAJ1
• Locus tag: CSC39_RS00240
• Protein ID: WP_001261274.1
• Coordinates: 31879..32109 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC39_RS00195	26634..26902	+	269	Protein_29	type II toxin-antitoxin system ParD family antitoxin	-
CSC39_RS00200	27018..27179	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSC39_RS00205	27246..27428	+	183	WP_042004909.1	hypothetical protein	-
CSC39_RS00210	27549..28289	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
CSC39_RS00215	28612..29601	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
CSC39_RS00220	30489..30758	+	270	WP_001275013.1	DUF1778 domain-containing protein	-
CSC39_RS00225	30762..31292	+	531	WP_000348883.1	GNAT family N-acetyltransferase	-
CSC39_RS00230	31293..31510	+	218	Protein_36	transposase	-
CSC39_RS00235	31515..31790	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	Toxin
CSC39_RS00240	31879..32109	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CSC39_RS00245	32308..34929	-	2622	WP_001586230.1	hypothetical protein	-
CSC39_RS00250	35320..35805	+	486	WP_001403958.1	hypothetical protein	-
CSC39_RS25700	35834..36606	+	773	Protein_41	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qepA4 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) / blaTEM-1B / tet(B)	-	1..128762	128762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• SpoVT-AbrB domain (7-45)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 9621.03 Da
• Isoelectric Point: 8.5444

>T107714 WP_001403920.1 NZ_CP030329:c31790-31515 [Escherichia coli]
VFSHRIVVSAITYSEMRFGASGPKASPRHVQLVDKFCPCLDAILSWDRAAVDGTTDIKVALRQAGTPIGQNDTAIAGPEA
GRLGKLTSPTL

Nucleotide

• Length: 276 bp

>T107714 NZ_CP030329:c31790-31515 [Escherichia coli]
GTGTTTAGTCACCGCATTGTGGTCTCGGCCATTACTTACTCTGAAATGCGCTTCGGTGCCTCCGGTCCGAAGGCCTCCCC
GCGTCACGTGCAACTGGTTGATAAATTCTGCCCGTGCCTCGATGCCATCCTGTCTTGGGACCGCGCCGCGGTGGACGGCA
CCACGGATATTAAAGTGGCACTGCGCCAGGCCGGGACGCCGATCGGCCAGAACGATACGGCGATTGCCGGGCCTGAAGCT
GGAAGACTGGGTAAATTAACATCGCCCACACTCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8856.95 Da
• Isoelectric Point: 4.6286

>AT107714 WP_001261274.1 NZ_CP030329:c32109-31879 [Escherichia coli]
MRTVSIFKNGNNRAIHLPRDLDFERVRELEIVREGDSIILRPVQPNWGSFAQLEKADPDFMAERENVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT107714 NZ_CP030329:c32109-31879 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATCCATCTGCCCCGCGATTTGGATTTTGAGCGGGTGAG
AGAGCTGGAGATCGTCCGCGAAGGAGACAGCATCATCCTGCGTCCCGTCCAGCCGAACTGGGGCTCATTCGCGCAGCTCG
AAAAAGCCGATCCGGATTTTATGGCGGAGCGCGAGAATGTTGTCAGCGACGAAGGTCGATTTGACCTGTGA

Structures

Antitoxin

• AlphaFold DB: G3CAJ1