Detailed information of TA system TA107713

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 30489..31292
• Replicon: plasmid unnamed1
• Accession: NZ_CP030329
• Organism: Escherichia coli strain AR_452

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: CSC39_RS00225
• Protein ID: WP_000348883.1
• Coordinates: 30762..31292 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: CSC39_RS00220
• Protein ID: WP_001275013.1
• Coordinates: 30489..30758 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC39_RS00185	25598..26338	+	741	WP_029702141.1	tyrosine-type recombinase/integrase	-
CSC39_RS00195	26634..26902	+	269	Protein_29	type II toxin-antitoxin system ParD family antitoxin	-
CSC39_RS00200	27018..27179	+	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CSC39_RS00205	27246..27428	+	183	WP_042004909.1	hypothetical protein	-
CSC39_RS00210	27549..28289	+	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
CSC39_RS00215	28612..29601	-	990	WP_123002471.1	RepB family plasmid replication initiator protein	-
CSC39_RS00220	30489..30758	+	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
CSC39_RS00225	30762..31292	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
CSC39_RS00230	31293..31510	+	218	Protein_36	transposase	-
CSC39_RS00235	31515..31790	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
CSC39_RS00240	31879..32109	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CSC39_RS00245	32308..34929	-	2622	WP_001586230.1	hypothetical protein	-
CSC39_RS00250	35320..35805	+	486	WP_001403958.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qepA4 / dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / mph(A) / blaTEM-1B / tet(B)	-	1..128762	128762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T107713 WP_000348883.1 NZ_CP030329:30762-31292 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T107713 NZ_CP030329:30762-31292 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT107713 WP_001275013.1 NZ_CP030329:30489-30758 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT107713 NZ_CP030329:30489-30758 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7