Detailed information of TA system TA107711

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 5933146..5934188
• Replicon: chromosome
• Accession: NZ_CP030327
• Organism: Pseudomonas aeruginosa strain AR_458

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CSC44_RS28460
• Protein ID: WP_003109777.1
• Coordinates: 5933613..5934188 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CSC44_RS28455
• Protein ID: WP_003050245.1
• Coordinates: 5933146..5933616 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC44_RS28420	5928538..5929956	-	1419	WP_003109776.1	TIGR03752 family integrating conjugative element protein	-
CSC44_RS28425	5929946..5930857	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
CSC44_RS28430	5930854..5931546	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
CSC44_RS28435	5931543..5931941	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
CSC44_RS28440	5931953..5932312	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CSC44_RS28445	5932329..5932562	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CSC44_RS28450	5932559..5932942	-	384	WP_003105635.1	RAQPRD family integrative conjugative element protein	-
CSC44_RS28455	5933146..5933616	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CSC44_RS28460	5933613..5934188	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
CSC44_RS28465	5934206..5935120	+	915	WP_112292640.1	AAA family ATPase	-
CSC44_RS28470	5935117..5935587	+	471	WP_003105626.1	hypothetical protein	-
CSC44_RS28475	5935584..5936084	+	501	WP_003090159.1	hypothetical protein	-
CSC44_RS28480	5936084..5936986	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
CSC44_RS28485	5937025..5937750	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T107711 WP_003109777.1 NZ_CP030327:5933613-5934188 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T107711 NZ_CP030327:5933613-5934188 [Pseudomonas aeruginosa]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT107711 WP_003050245.1 NZ_CP030327:5933146-5933616 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT107711 NZ_CP030327:5933146-5933616 [Pseudomonas aeruginosa]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68