Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /HTH_XRE(antitoxin)
Location 362728..363525 Replicon chromosome
Accession NZ_CP030326
Organism Staphylococcus aureus strain AR_474

Toxin (Protein)


Gene name - Uniprot ID A0A6B0AWK0
Locus tag CSC59_RS01845 Protein ID WP_000525016.1
Coordinates 362728..363189 (-) Length 154 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A0C2LD36
Locus tag CSC59_RS01850 Protein ID WP_001260487.1
Coordinates 363202..363525 (-) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CSC59_RS01825 358567..360501 + 1935 Protein_309 YSIRK domain-containing triacylglycerol lipase Lip2/Geh -
CSC59_RS01830 360595..361800 - 1206 WP_000264186.1 site-specific integrase -
CSC59_RS01835 361911..362090 + 180 WP_000337827.1 hypothetical protein -
CSC59_RS01840 362216..362707 - 492 WP_001077638.1 hypothetical protein -
CSC59_RS01845 362728..363189 - 462 WP_000525016.1 hypothetical protein Toxin
CSC59_RS01850 363202..363525 - 324 WP_001260487.1 helix-turn-helix domain-containing protein Antitoxin
CSC59_RS01855 363689..363937 + 249 WP_000272858.1 helix-turn-helix domain-containing protein -
CSC59_RS15530 363952..364113 + 162 WP_001153175.1 hypothetical protein -
CSC59_RS01860 364106..364342 - 237 WP_000856472.1 hypothetical protein -
CSC59_RS01865 364793..364978 + 186 WP_000933365.1 helix-turn-helix transcriptional regulator -
CSC59_RS01870 364980..365732 + 753 WP_001148634.1 phage antirepressor KilAC domain-containing protein -
CSC59_RS01875 365748..365966 + 219 WP_000993180.1 hypothetical protein -
CSC59_RS01880 365996..366259 + 264 WP_001124198.1 helix-turn-helix domain-containing protein -
CSC59_RS01885 366271..366432 + 162 WP_000066020.1 DUF1270 domain-containing protein -
CSC59_RS01890 366527..366829 + 303 WP_000165371.1 DUF2482 family protein -
CSC59_RS01895 366834..367094 + 261 WP_000291510.1 DUF1108 family protein -
CSC59_RS01900 367103..367366 + 264 WP_001205732.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 360595..402858 42263


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(8-65)


Sequences

Toxin        


Download         Length: 154 a.a.        Molecular weight: 17993.29 Da        Isoelectric Point: 4.5941

>T107687 WP_000525016.1 NZ_CP030326:c363189-362728 [Staphylococcus aureus]
MGLYEETLIQHDYIEVREADVLPDNLDGVWLGDLILIKRGLSDTEKAGILFEELAHNKLTYGDIADYSKFNNRKFENYAR
RHGFTSAVPIREIVEAYNYGVRNLYELSEYLQLSEEYILEAIEQYKKIYGIGTHYGEYSITFEPLRVFKYKEI

Download         Length: 462 bp

>T107687 NZ_CP030326:c363189-362728 [Staphylococcus aureus]
ATGGGATTATATGAAGAAACTTTAATACAACATGATTATATTGAAGTAAGAGAGGCTGATGTACTTCCAGATAATTTAGA
CGGGGTATGGTTAGGAGATCTAATTTTAATAAAGCGTGGTTTATCAGATACAGAAAAGGCAGGAATTCTCTTCGAAGAAT
TAGCACATAATAAACTTACATACGGTGATATAGCTGATTACTCGAAATTCAACAATCGCAAGTTCGAAAATTATGCACGT
AGACATGGTTTTACTTCAGCTGTACCGATACGTGAAATTGTAGAAGCTTACAATTATGGCGTACGTAACTTGTATGAGTT
GTCTGAGTATCTACAATTAAGCGAAGAATACATATTGGAGGCAATAGAACAATATAAGAAGATATACGGTATTGGAACTC
ACTATGGCGAGTATTCGATTACATTTGAGCCGTTGAGAGTTTTTAAATATAAAGAAATATGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12431.09 Da        Isoelectric Point: 9.3811

>AT107687 WP_001260487.1 NZ_CP030326:c363525-363202 [Staphylococcus aureus]
MRTSAEIGKLIKQLRKENNVNLTDFATKIGVNKSTLSRYENGSRKIPMEDIAEIANALKVTPEYLLLKNRQTENEVQHRA
AHLEGELTDDEWQRVLDYADYIRSKRK

Download         Length: 324 bp

>AT107687 NZ_CP030326:c363525-363202 [Staphylococcus aureus]
ATGAGAACTTCAGCAGAAATAGGTAAATTAATCAAACAACTACGAAAAGAGAATAATGTGAATTTAACTGATTTTGCAAC
TAAGATAGGTGTCAATAAATCTACCTTATCCCGATATGAAAACGGTAGCAGAAAAATACCTATGGAGGATATAGCTGAAA
TTGCCAATGCATTGAAAGTTACCCCAGAATATTTACTATTAAAAAATAGACAAACAGAAAACGAAGTACAACATCGAGCA
GCTCACCTTGAAGGAGAATTGACAGATGATGAATGGCAAAGAGTTTTAGATTATGCAGATTATATAAGAAGTAAACGTAA
GTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6B0AWK0

Antitoxin

Source ID Structure
AlphaFold DB A0A0C2LD36

References