Detailed information of TA system TA107679

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1194822..1195351
• Replicon: chromosome
• Accession: NZ_CP030323
• Organism: Staphylococcus aureus strain AR_475

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CSC60_RS05970
• Protein ID: WP_000621175.1
• Coordinates: 1194989..1195351 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: CSC60_RS05965
• Protein ID: WP_000948331.1
• Coordinates: 1194822..1194992 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CSC60_RS05935	1189858..1190418	+	561	WP_001092405.1	K(+)-transporting ATPase subunit C	-
CSC60_RS05940	1190627..1191106	+	480	WP_001287083.1	hypothetical protein	-
CSC60_RS05945	1191099..1192682	+	1584	WP_001294642.1	PH domain-containing protein	-
CSC60_RS05950	1192669..1193160	+	492	WP_001205915.1	PH domain-containing protein	-
CSC60_RS05955	1193164..1193523	+	360	WP_000581194.1	holo-ACP synthase	-
CSC60_RS05960	1193589..1194737	+	1149	WP_001280702.1	alanine racemase	-
CSC60_RS05965	1194822..1194992	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
CSC60_RS05970	1194989..1195351	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CSC60_RS05980	1195700..1196701	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
CSC60_RS05985	1196820..1197146	+	327	WP_001052491.1	anti-sigma factor antagonist	-
CSC60_RS05990	1197148..1197627	+	480	WP_001190825.1	anti-sigma B factor RsbW	-
CSC60_RS05995	1197602..1198372	+	771	WP_001041107.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T107679 WP_000621175.1 NZ_CP030323:1194989-1195351 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T107679 NZ_CP030323:1194989-1195351 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT107679 WP_000948331.1 NZ_CP030323:1194822-1194992 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT107679 NZ_CP030323:1194822-1194992 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1