Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 105640..106376 Replicon plasmid p23-1
Accession NZ_CP030321
Organism Klebsiella pneumoniae strain 23

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag DRA87_RS26075 Protein ID WP_004098919.1
Coordinates 105894..106376 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag DRA87_RS26070 Protein ID WP_004213599.1
Coordinates 105640..105906 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRA87_RS26045 (DRA87_26880) 100872..101429 + 558 WP_004213590.1 recombinase family protein -
DRA87_RS26050 (DRA87_26885) 101432..104401 + 2970 WP_094936121.1 Tn3 family transposase -
DRA87_RS26055 (DRA87_26890) 104443..104937 + 495 WP_004213594.1 hypothetical protein -
DRA87_RS26060 (DRA87_26895) 104998..105201 + 204 WP_004213596.1 HHA domain-containing protein -
DRA87_RS26065 (DRA87_26900) 105215..105445 + 231 WP_004213598.1 hypothetical protein -
DRA87_RS26070 (DRA87_26905) 105640..105906 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
DRA87_RS26075 (DRA87_26910) 105894..106376 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
DRA87_RS26080 (DRA87_26915) 106797..108024 + 1228 Protein_109 IS3 family transposase -
DRA87_RS26085 (DRA87_26920) 108020..108415 - 396 Protein_110 IS3 family transposase -
DRA87_RS26090 (DRA87_26930) 108590..109612 - 1023 WP_004214536.1 porphobilinogen synthase -
DRA87_RS26095 (DRA87_26935) 109676..111022 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA / rmpA / pla 1..203046 203046
- inside IScluster/Tn - - 100872..108024 7152


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T107669 WP_004098919.1 NZ_CP030321:105894-106376 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T107669 NZ_CP030321:105894-106376 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT107669 WP_004213599.1 NZ_CP030321:105640-105906 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT107669 NZ_CP030321:105640-105906 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References