Detailed information of TA system TA107668

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 40237..40907
• Replicon: plasmid p23-1
• Accession: NZ_CP030321
• Organism: Klebsiella pneumoniae strain 23

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DRA87_RS25740
• Protein ID: WP_004213072.1
• Coordinates: 40464..40907 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DRA87_RS25735
• Protein ID: WP_004213073.1
• Coordinates: 40237..40467 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRA87_RS25710 (DRA87_26510)	36460..37359	-	900	WP_004225022.1	nucleotide-binding protein	-
DRA87_RS25715 (DRA87_26515)	37349..37639	-	291	WP_004213078.1	hypothetical protein	-
DRA87_RS25720 (DRA87_26520)	37991..38197	+	207	WP_004213077.1	hypothetical protein	-
DRA87_RS25725 (DRA87_26525)	38187..38480	-	294	WP_004213076.1	hypothetical protein	-
DRA87_RS25730 (DRA87_26530)	38496..39629	-	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DRA87_RS25735 (DRA87_26535)	40237..40467	+	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DRA87_RS25740 (DRA87_26540)	40464..40907	+	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DRA87_RS27335	41056..41307	+	252	WP_186987481.1	hypothetical protein	-
DRA87_RS25750 (DRA87_26550)	41330..41634	-	305	Protein_43	transposase	-
DRA87_RS25755	42051..42686	+	636	WP_165452888.1	mucoid phenotype regulator RmpA2	-
DRA87_RS25760 (DRA87_26570)	43204..43607	-	404	Protein_45	GAF domain-containing protein	-
DRA87_RS25765 (DRA87_26580)	43698..44618	-	921	WP_004213934.1	DUF1471 domain-containing protein	-
DRA87_RS25770 (DRA87_26585)	44667..45158	-	492	WP_011251327.1	DM13 domain-containing protein	-
DRA87_RS25775 (DRA87_26590)	45221..45496	-	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / iroB / iroC / iroD / iroN / rmpA / rmpA / pla	1..203046	203046

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T107668 WP_004213072.1 NZ_CP030321:40464-40907 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T107668 NZ_CP030321:40464-40907 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT107668 WP_004213073.1 NZ_CP030321:40237-40467 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT107668 NZ_CP030321:40237-40467 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620