Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4728203..4728719 Replicon chromosome
Accession NZ_CP030320
Organism Klebsiella pneumoniae strain 23

Toxin (Protein)


Gene name relE Uniprot ID R4YAY3
Locus tag DRA87_RS22865 Protein ID WP_004178374.1
Coordinates 4728203..4728487 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID R4Y888
Locus tag DRA87_RS22870 Protein ID WP_002886901.1
Coordinates 4728477..4728719 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRA87_RS22840 (DRA87_23455) 4723599..4723862 - 264 WP_004152271.1 PTS sugar transporter subunit IIB -
DRA87_RS22845 4723992..4724165 + 174 WP_004222159.1 hypothetical protein -
DRA87_RS22850 (DRA87_23460) 4724168..4724911 + 744 WP_002886905.1 MurR/RpiR family transcriptional regulator -
DRA87_RS22855 (DRA87_23465) 4725268..4727406 + 2139 WP_032415880.1 anaerobic ribonucleoside-triphosphate reductase -
DRA87_RS22860 (DRA87_23470) 4727735..4728199 + 465 WP_004178375.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
DRA87_RS22865 (DRA87_23475) 4728203..4728487 - 285 WP_004178374.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DRA87_RS22870 (DRA87_23480) 4728477..4728719 - 243 WP_002886901.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
DRA87_RS22875 (DRA87_23485) 4728797..4730707 - 1911 WP_130974884.1 PRD domain-containing protein -
DRA87_RS22880 (DRA87_23490) 4730730..4731884 - 1155 WP_004178372.1 lactonase family protein -
DRA87_RS22885 (DRA87_23495) 4731951..4732691 - 741 WP_004186692.1 KDGP aldolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-80)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11155.97 Da        Isoelectric Point: 10.3787

>T107665 WP_004178374.1 NZ_CP030320:c4728487-4728203 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Download         Length: 285 bp

>T107665 NZ_CP030320:c4728487-4728203 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9045.55 Da        Isoelectric Point: 6.3340

>AT107665 WP_002886901.1 NZ_CP030320:c4728719-4728477 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Download         Length: 243 bp

>AT107665 NZ_CP030320:c4728719-4728477 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCTGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A6THG1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLP0

References