Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4007391..4008010 Replicon chromosome
Accession NZ_CP030320
Organism Klebsiella pneumoniae strain 23

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag DRA87_RS19490 Protein ID WP_002892050.1
Coordinates 4007792..4008010 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag DRA87_RS19485 Protein ID WP_002892066.1
Coordinates 4007391..4007765 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRA87_RS19475 (DRA87_19970) 4002543..4003736 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DRA87_RS19480 (DRA87_19975) 4003759..4006905 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
DRA87_RS19485 (DRA87_19980) 4007391..4007765 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
DRA87_RS19490 (DRA87_19985) 4007792..4008010 + 219 WP_002892050.1 HHA domain-containing protein Toxin
DRA87_RS19495 (DRA87_19990) 4008169..4008735 + 567 WP_002892030.1 maltose O-acetyltransferase -
DRA87_RS19500 4008707..4008847 - 141 WP_004147370.1 hypothetical protein -
DRA87_RS19505 (DRA87_19995) 4008868..4009338 + 471 WP_002892026.1 YlaC family protein -
DRA87_RS19510 (DRA87_20000) 4009313..4010764 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
DRA87_RS19515 (DRA87_20005) 4010865..4011563 + 699 WP_032414604.1 GNAT family protein -
DRA87_RS19520 (DRA87_20010) 4011560..4011700 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
DRA87_RS19525 (DRA87_20015) 4011700..4011963 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107663 WP_002892050.1 NZ_CP030320:4007792-4008010 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T107663 NZ_CP030320:4007792-4008010 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT107663 WP_002892066.1 NZ_CP030320:4007391-4007765 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT107663 NZ_CP030320:4007391-4007765 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References