Detailed information of TA system TA107650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 199506..200245
• Replicon: chromosome
• Accession: NZ_CP030320
• Organism: Klebsiella pneumoniae strain 23

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DRA87_RS00975
• Protein ID: WP_021312536.1
• Coordinates: 199760..200245 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: DRA87_RS00970
• Protein ID: WP_003026799.1
• Coordinates: 199506..199772 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRA87_RS00955 (DRA87_00965)	195009..197078	+	2070	WP_002922127.1	glycine--tRNA ligase subunit beta	-
DRA87_RS00960 (DRA87_00970)	197375..198744	+	1370	WP_087830479.1	IS3 family transposase	-
DRA87_RS00965 (DRA87_00975)	198945..199373	+	429	WP_004901287.1	GFA family protein	-
DRA87_RS00970 (DRA87_00980)	199506..199772	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
DRA87_RS00975 (DRA87_00985)	199760..200245	+	486	WP_021312536.1	GNAT family N-acetyltransferase	Toxin
DRA87_RS00980 (DRA87_00990)	200589..200741	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
DRA87_RS00985 (DRA87_00995)	201043..202662	+	1620	WP_032415799.1	ABC-F family ATP-binding cassette domain-containing protein	-
DRA87_RS00990 (DRA87_01000)	202761..202973	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
DRA87_RS00995 (DRA87_01005)	203226..203516	-	291	WP_002921931.1	HTH-type transcriptional regulator	-
DRA87_RS01000 (DRA87_01010)	203762..205117	-	1356	WP_032415797.1	aromatic acid/H+ symport family MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	198019..198744	725

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17608.39 Da
• Isoelectric Point: 10.3370

>T107650 WP_021312536.1 NZ_CP030320:199760-200245 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T107650 NZ_CP030320:199760-200245 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT107650 WP_003026799.1 NZ_CP030320:199506-199772 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT107650 NZ_CP030320:199506-199772 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66