Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5230599..5231224 Replicon chromosome
Accession NZ_CP030315
Organism Klebsiella pneumoniae strain 11305

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DRB03_RS25495 Protein ID WP_002882817.1
Coordinates 5230599..5230982 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DRB03_RS25500 Protein ID WP_004150355.1
Coordinates 5230982..5231224 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB03_RS25480 (5227965) 5227965..5228867 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DRB03_RS25485 (5228864) 5228864..5229499 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DRB03_RS25490 (5229496) 5229496..5230425 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DRB03_RS25495 (5230599) 5230599..5230982 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DRB03_RS25500 (5230982) 5230982..5231224 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DRB03_RS25505 (5231429) 5231429..5232346 + 918 WP_021466676.1 alpha/beta hydrolase -
DRB03_RS25510 (5232360) 5232360..5233301 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DRB03_RS25515 (5233346) 5233346..5233783 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DRB03_RS25520 (5233780) 5233780..5234640 - 861 WP_004146232.1 virulence factor BrkB family protein -
DRB03_RS25525 (5234634) 5234634..5235233 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T107649 WP_002882817.1 NZ_CP030315:c5230982-5230599 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107649 NZ_CP030315:c5230982-5230599 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107649 WP_004150355.1 NZ_CP030315:c5231224-5230982 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107649 NZ_CP030315:c5231224-5230982 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References