Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1471059..1471795 Replicon chromosome
Accession NZ_CP030315
Organism Klebsiella pneumoniae strain 11305

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag DRB03_RS07265 Protein ID WP_032433360.1
Coordinates 1471313..1471795 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag DRB03_RS07260 Protein ID WP_003026799.1
Coordinates 1471059..1471325 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB03_RS07235 (1466705) 1466705..1467844 + 1140 WP_032433368.1 mannitol dehydrogenase -
DRB03_RS07240 (1467873) 1467873..1468535 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
DRB03_RS07245 (1468519) 1468519..1469523 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
DRB03_RS07250 (1469541) 1469541..1470173 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
DRB03_RS07255 (1470183) 1470183..1470746 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
DRB03_RS07260 (1471059) 1471059..1471325 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
DRB03_RS07265 (1471313) 1471313..1471795 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
DRB03_RS07270 (1472156) 1472156..1472755 - 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
DRB03_RS07275 (1472968) 1472968..1473912 - 945 WP_077254249.1 fimbrial protein -
DRB03_RS07280 (1473924) 1473924..1474502 - 579 WP_032432061.1 type 1 fimbrial protein -
DRB03_RS07285 (1474506) 1474506..1475246 - 741 WP_050597964.1 molecular chaperone -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1441282..1482738 41456


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T107635 WP_032433360.1 NZ_CP030315:1471313-1471795 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T107635 NZ_CP030315:1471313-1471795 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT107635 WP_003026799.1 NZ_CP030315:1471059-1471325 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT107635 NZ_CP030315:1471059-1471325 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References