Detailed information of TA system TA107629

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 53160..53830
• Replicon: plasmid p11311-1
• Accession: NZ_CP030314
• Organism: Klebsiella pneumoniae strain 11311

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: DRA79_RS25630
• Protein ID: WP_004213072.1
• Coordinates: 53160..53603 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: DRA79_RS25635
• Protein ID: WP_004213073.1
• Coordinates: 53600..53830 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRA79_RS25595 (DRA79_26400)	48572..48847	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
DRA79_RS25600 (DRA79_26405)	48910..49401	+	492	WP_011251327.1	DM13 domain-containing protein	-
DRA79_RS25605 (DRA79_26410)	49450..50370	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
DRA79_RS25610 (DRA79_26420)	50461..50864	+	404	Protein_54	GAF domain-containing protein	-
DRA79_RS25615	51381..52016	-	636	WP_165452888.1	mucoid phenotype regulator RmpA2	-
DRA79_RS25620 (DRA79_26440)	52433..52737	+	305	Protein_56	transposase	-
DRA79_RS26555	52760..53011	-	252	WP_186987481.1	hypothetical protein	-
DRA79_RS25630 (DRA79_26450)	53160..53603	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
DRA79_RS25635 (DRA79_26455)	53600..53830	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DRA79_RS25640 (DRA79_26460)	54438..55571	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
DRA79_RS25645 (DRA79_26465)	55587..55880	+	294	WP_004213076.1	hypothetical protein	-
DRA79_RS25650 (DRA79_26470)	55870..56076	-	207	WP_004213077.1	hypothetical protein	-
DRA79_RS25655 (DRA79_26475)	56428..56718	+	291	WP_004213078.1	hypothetical protein	-
DRA79_RS25660 (DRA79_26480)	56708..57607	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iroB / iroC / iroD / iroN / rmpA / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA / pla	1..203044	203044

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T107629 WP_004213072.1 NZ_CP030314:c53603-53160 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T107629 NZ_CP030314:c53603-53160 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT107629 WP_004213073.1 NZ_CP030314:c53830-53600 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT107629 NZ_CP030314:c53830-53600 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620