Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5191977..5192602 Replicon chromosome
Accession NZ_CP030313
Organism Klebsiella pneumoniae strain 11311

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DRA79_RS24980 Protein ID WP_002882817.1
Coordinates 5191977..5192360 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DRA79_RS24985 Protein ID WP_004150355.1
Coordinates 5192360..5192602 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRA79_RS24965 (DRA79_25710) 5189343..5190245 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DRA79_RS24970 (DRA79_25715) 5190242..5190877 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DRA79_RS24975 (DRA79_25720) 5190874..5191803 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DRA79_RS24980 (DRA79_25725) 5191977..5192360 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DRA79_RS24985 (DRA79_25730) 5192360..5192602 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DRA79_RS24990 (DRA79_25735) 5192807..5193724 + 918 WP_012737090.1 alpha/beta hydrolase -
DRA79_RS24995 (DRA79_25740) 5193738..5194679 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
DRA79_RS25000 (DRA79_25745) 5194724..5195161 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DRA79_RS25005 (DRA79_25750) 5195158..5196018 - 861 WP_004146232.1 virulence factor BrkB family protein -
DRA79_RS25010 (DRA79_25755) 5196012..5196611 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T107627 WP_002882817.1 NZ_CP030313:c5192360-5191977 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107627 NZ_CP030313:c5192360-5191977 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107627 WP_004150355.1 NZ_CP030313:c5192602-5192360 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107627 NZ_CP030313:c5192602-5192360 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATACCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References