Detailed information of TA system TA107609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 194472..195388
• Replicon: plasmid p11454-1
• Accession: NZ_CP030312
• Organism: Klebsiella pneumoniae strain 11454

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: DRA80_RS26015
• Protein ID: WP_114444169.1
• Coordinates: 194912..195388 (+)
• Length: 159 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: DRA80_RS26010
• Protein ID: WP_004026357.1
• Coordinates: 194472..194915 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRA80_RS25980 (DRA80_26815)	189490..190011	+	522	WP_004213635.1	ferric citrate uptake sigma factor FecI	-
DRA80_RS25985 (DRA80_26820)	190008..190961	+	954	Protein_201	fec operon regulator FecR	-
DRA80_RS25990 (DRA80_26825)	191048..193174	+	2127	WP_004213640.1	TonB-dependent Fe(3+) dicitrate receptor FecA	-
DRA80_RS25995 (DRA80_26830)	193249..193368	+	120	Protein_203	Fe3+-citrate ABC transporter substrate-binding protein	-
DRA80_RS26000 (DRA80_26835)	193549..193770	+	222	WP_004213643.1	DUF2188 domain-containing protein	-
DRA80_RS26005 (DRA80_26840)	193868..194371	+	504	Protein_205	DUF4113 domain-containing protein	-
DRA80_RS26010 (DRA80_26845)	194472..194915	+	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
DRA80_RS26015 (DRA80_26850)	194912..195388	+	477	WP_114444169.1	RES family NAD+ phosphorylase	Toxin
DRA80_RS26020 (DRA80_26855)	195331..195510	-	180	Protein_208	IS3 family transposase	-
DRA80_RS26025 (DRA80_26865)	195565..196262	+	698	WP_223175001.1	IS1-like element IS1A family transposase	-
DRA80_RS26030 (DRA80_26870)	197648..198298	-	651	WP_068893702.1	DUF1173 family protein	-
DRA80_RS26300	198331..198597	-	267	WP_223175074.1	DUF1173 family protein	-
DRA80_RS26035 (DRA80_26875)	198775..199269	+	495	WP_004212794.1	thermonuclease family protein	-
DRA80_RS26040 (DRA80_26880)	199606..200004	+	399	WP_004212796.1	H-NS family nucleoid-associated regulatory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..211169	211169
	-	flank	IS/Tn	-	-	195759..196262	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-138)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 159 a.a.
• Molecular weight: 17743.18 Da
• Isoelectric Point: 4.7156

>T107609 WP_114444169.1 NZ_CP030312:194912-195388 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIVWVHHILQFLLSRKI

Nucleotide

• Length: 477 bp

>T107609 NZ_CP030312:194912-195388 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATTGTATGGGTCCACCACATATTGCAATTTTTACTCTCACGTAAAATATAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT107609 WP_004026357.1 NZ_CP030312:194472-194915 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT107609 NZ_CP030312:194472-194915 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6P1V3Q9