Detailed information of TA system TA107607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 20708..21444
• Replicon: plasmid p11454-1
• Accession: NZ_CP030312
• Organism: Klebsiella pneumoniae strain 11454

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: DRA80_RS25095
• Protein ID: WP_004213601.1
• Coordinates: 20708..21190 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: DRA80_RS25100
• Protein ID: WP_004213599.1
• Coordinates: 21178..21444 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRA80_RS25075 (DRA80_25840)	16062..17408	+	1347	WP_011154555.1	dihydroorotase	-
DRA80_RS25080 (DRA80_25845)	17472..18494	+	1023	WP_004214536.1	porphobilinogen synthase	-
DRA80_RS25085 (DRA80_25855)	18669..19064	+	396	Protein_19	IS3 family transposase	-
DRA80_RS25090 (DRA80_25860)	19060..20287	-	1228	Protein_20	IS3 family transposase	-
DRA80_RS25095 (DRA80_25865)	20708..21190	-	483	WP_004213601.1	GNAT family N-acetyltransferase	Toxin
DRA80_RS25100 (DRA80_25870)	21178..21444	-	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
DRA80_RS25105 (DRA80_25875)	21639..21869	-	231	WP_004213598.1	hypothetical protein	-
DRA80_RS25110 (DRA80_25880)	21883..22086	-	204	WP_004213596.1	HHA domain-containing protein	-
DRA80_RS25115 (DRA80_25885)	22147..22641	-	495	WP_004213594.1	hypothetical protein	-
DRA80_RS25120 (DRA80_25890)	22683..25652	-	2970	WP_004213592.1	Tn3 family transposase	-
DRA80_RS25125 (DRA80_25895)	25655..26212	-	558	WP_004213590.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iutA / iucD / iucC / iucB / iucA / rmpA / iroN / iroD / iroC / iroB	1..211169	211169
	-	inside	IScluster/Tn	-	-	19060..26212	7152

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17307.01 Da
• Isoelectric Point: 10.0704

>T107607 WP_004213601.1 NZ_CP030312:c21190-20708 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETALDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T107607 NZ_CP030312:c21190-20708 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGCCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT107607 WP_004213599.1 NZ_CP030312:c21444-21178 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT107607 NZ_CP030312:c21444-21178 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0