Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5121173..5121798 Replicon chromosome
Accession NZ_CP030311
Organism Klebsiella pneumoniae strain 11454

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DRA80_RS24630 Protein ID WP_002882817.1
Coordinates 5121173..5121556 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DRA80_RS24635 Protein ID WP_004150355.1
Coordinates 5121556..5121798 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRA80_RS24615 (5118539) 5118539..5119441 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DRA80_RS24620 (5119438) 5119438..5120073 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DRA80_RS24625 (5120070) 5120070..5120999 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DRA80_RS24630 (5121173) 5121173..5121556 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DRA80_RS24635 (5121556) 5121556..5121798 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DRA80_RS24640 (5122003) 5122003..5122920 + 918 WP_004213381.1 alpha/beta hydrolase -
DRA80_RS24645 (5122935) 5122935..5123876 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DRA80_RS24650 (5123921) 5123921..5124358 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DRA80_RS24655 (5124355) 5124355..5125215 - 861 WP_004146232.1 virulence factor BrkB family protein -
DRA80_RS24660 (5125209) 5125209..5125808 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T107606 WP_002882817.1 NZ_CP030311:c5121556-5121173 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107606 NZ_CP030311:c5121556-5121173 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACTTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107606 WP_004150355.1 NZ_CP030311:c5121798-5121556 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107606 NZ_CP030311:c5121798-5121556 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References