Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1727353..1727943 Replicon chromosome
Accession NZ_CP030311
Organism Klebsiella pneumoniae strain 11454

Toxin (Protein)


Gene name pemK Uniprot ID A0A2L1BXX2
Locus tag DRA80_RS08310 Protein ID WP_022615588.1
Coordinates 1727611..1727943 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A3S7DDD0
Locus tag DRA80_RS08305 Protein ID WP_000288812.1
Coordinates 1727353..1727610 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRA80_RS26160 (1723261) 1723261..1723338 + 78 Protein_1623 type II toxin-antitoxin system PemK/MazF family toxin -
DRA80_RS08285 (1723688) 1723688..1725436 + 1749 WP_032457402.1 hypothetical protein -
DRA80_RS08290 (1725515) 1725515..1726071 + 557 Protein_1625 hypothetical protein -
DRA80_RS08295 (1726354) 1726354..1726815 + 462 WP_004213450.1 hypothetical protein -
DRA80_RS08300 (1726812) 1726812..1727018 + 207 WP_022615589.1 helix-turn-helix transcriptional regulator -
DRA80_RS08305 (1727353) 1727353..1727610 + 258 WP_000288812.1 antitoxin Antitoxin
DRA80_RS08310 (1727611) 1727611..1727943 + 333 WP_022615588.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
DRA80_RS08320 (1728265) 1728265..1729701 + 1437 WP_004151469.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
DRA80_RS08330 (1730067) 1730067..1731521 - 1455 WP_004148975.1 AMP nucleosidase -
DRA80_RS08335 (1731651) 1731651..1731896 - 246 WP_004141189.1 signal transduction protein PmrD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11913.80 Da        Isoelectric Point: 10.1839

>T107592 WP_022615588.1 NZ_CP030311:1727611-1727943 [Klebsiella pneumoniae]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDVVVNEVLARLDAMLS

Download         Length: 333 bp

>T107592 NZ_CP030311:1727611-1727943 [Klebsiella pneumoniae]
ATGGACAGAGGGGAAATCTGGCTTGTTTCACTGGATCCGATTGCTGGCCACGAGCAAAGTGGAAAACGTCCGGTACTCAT
TGTATCGAAGGCATCGTTTAACAAGCTGACCCGACTACCGGTCGTGGTTCCCGTCACCAGCGGCGGAAACTTTGCACGTA
CAGCAGGTTTCACGGTTTCACTGGAAGAGGCGGGGACCAAAACAACAGGCGTTATTCGCTGCGACCAGCCCAGGACTATC
GATATGGCGGCCCGGAACGGCAAGCGTCTGGAACGCATACCTGACGTCGTTGTCAACGAGGTGCTTGCCAGGCTGGATGC
GATGCTGAGTTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9442.92 Da        Isoelectric Point: 4.3566

>AT107592 WP_000288812.1 NZ_CP030311:1727353-1727610 [Klebsiella pneumoniae]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT107592 NZ_CP030311:1727353-1727610 [Klebsiella pneumoniae]
ATGTATACAACTCGCCTGAAAAAGGTAGGCGGATCCATCATGCTGGCGGTTCCTCCCGCCGTGCTGAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTGGGTATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1BXX2

Antitoxin

Source ID Structure
AlphaFold DB A0A3S7DDD0

References