Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 23930..24489 Replicon plasmid p205880-5
Accession NZ_CP030307
Organism Klebsiella pneumoniae strain 205880

Toxin (Protein)


Gene name relE Uniprot ID S1PMX5
Locus tag DRB04_RS28700 Protein ID WP_000421257.1
Coordinates 23930..24205 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A5I3EQ05
Locus tag DRB04_RS28705 Protein ID WP_001674495.1
Coordinates 24205..24489 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB04_RS28675 (19607) 19607..19921 - 315 WP_001302628.1 hypothetical protein -
DRB04_RS28680 (20491) 20491..20748 + 258 WP_094308825.1 hypothetical protein -
DRB04_RS28685 (20748) 20748..21338 + 591 WP_102000831.1 hypothetical protein -
DRB04_RS28690 (21550) 21550..22602 + 1053 WP_000655519.1 hypothetical protein -
DRB04_RS28695 (22778) 22778..23380 + 603 WP_094308828.1 ProQ/FINO family protein -
DRB04_RS30495 (23436) 23436..23675 + 240 Protein_33 hypothetical protein -
DRB04_RS28700 (23930) 23930..24205 - 276 WP_000421257.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DRB04_RS28705 (24205) 24205..24489 - 285 WP_001674495.1 ribbon-helix-helix domain-containing protein Antitoxin
DRB04_RS28710 (25394) 25394..26425 - 1032 WP_000907867.1 plasmid replication initiator RepA -
DRB04_RS28715 (27429) 27429..27659 + 231 WP_228772074.1 ash family protein -
DRB04_RS28720 (27577) 27577..27864 + 288 WP_000074849.1 conjugal transfer protein TraA -
DRB04_RS28725 (28306) 28306..28839 + 534 WP_001306770.1 transcription termination/antitermination NusG family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..82822 82822


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 9.9324

>T107584 WP_000421257.1 NZ_CP030307:c24205-23930 [Klebsiella pneumoniae]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T107584 NZ_CP030307:c24205-23930 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT107584 WP_001674495.1 NZ_CP030307:c24489-24205 [Klebsiella pneumoniae]
MQMKNNSAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT107584 NZ_CP030307:c24489-24205 [Klebsiella pneumoniae]
ATGCAAATGAAGAACAATAGTGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCTCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PMX5

Antitoxin

Source ID Structure
AlphaFold DB A0A5I3EQ05

References