Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 39320..39845 Replicon plasmid p205880-3
Accession NZ_CP030305
Organism Klebsiella pneumoniae strain 205880

Toxin (Protein)


Gene name ccdB Uniprot ID W8EAA6
Locus tag DRB04_RS27575 Protein ID WP_008322213.1
Coordinates 39320..39625 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A6C0NE25
Locus tag DRB04_RS27580 Protein ID WP_032934863.1
Coordinates 39627..39845 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB04_RS27545 (DRB04_28420) 34457..34573 + 117 Protein_45 IS5 family transposase -
DRB04_RS27550 (DRB04_28430) 34645..35765 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -
DRB04_RS27555 (DRB04_28435) 35794..36540 + 747 Protein_47 IS5-like element IS5 family transposase -
DRB04_RS27560 (DRB04_28445) 36684..38024 + 1341 Protein_48 GntP family transporter -
DRB04_RS27565 (DRB04_28450) 38223..39014 - 792 WP_008322208.1 site-specific integrase -
DRB04_RS27570 (DRB04_28455) 39004..39318 - 315 WP_008322211.1 hypothetical protein -
DRB04_RS27575 (DRB04_28460) 39320..39625 - 306 WP_008322213.1 type II toxin-antitoxin system toxin CcdB Toxin
DRB04_RS27580 (DRB04_28465) 39627..39845 - 219 WP_032934863.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DRB04_RS27585 (DRB04_28470) 40707..41404 - 698 Protein_53 IS1-like element IS1B family transposase -
DRB04_RS27590 (DRB04_28480) 41617..44199 + 2583 WP_016479955.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..110786 110786
- inside IScluster/Tn - - 28296..56490 28194


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11450.23 Da        Isoelectric Point: 5.8211

>T107583 WP_008322213.1 NZ_CP030305:c39625-39320 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLVSARLLSDKVSRELYPVVSVGGDPYRLMTTDMASVPATVIGEEV
ADLSHLENDIQNAINLMFRGI

Download         Length: 306 bp

>T107583 NZ_CP030305:c39625-39320 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAAAGCCGCTACCGTCTGTTCGTCGACGTGCAGAGTGACATTATTGACAC
CCCGGGACGCCGGATGGTGATCCCGCTGGTCAGCGCCCGCCTGCTGTCCGACAAAGTCTCGCGTGAGCTTTATCCGGTGG
TGTCAGTCGGAGGTGACCCTTATCGTCTGATGACCACGGATATGGCCAGTGTGCCGGCCACTGTCATCGGGGAAGAAGTG
GCCGATCTCAGCCACCTGGAGAATGACATCCAGAACGCCATCAACCTGATGTTCCGGGGGATCTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8297.11 Da        Isoelectric Point: 4.4013

>AT107583 WP_032934863.1 NZ_CP030305:c39845-39627 [Klebsiella pneumoniae]
VKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQEENREGMAEVASFIEANGSFADENRDW

Download         Length: 219 bp

>AT107583 NZ_CP030305:c39845-39627 [Klebsiella pneumoniae]
GTGAAGCAGCGTATTACAGTGACAGTGGACAGCGACAGCTATCAGTTGCTCAAGGCATACGACGTAAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGCCGTCTGCGCGCCGAACGCTGGCAGGAAGAAAACCGGGAAGGTATGGCTG
AGGTGGCCAGCTTTATCGAGGCTAACGGATCGTTTGCTGACGAAAACAGGGATTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0NE27

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE25

References