Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 30256..31059 Replicon plasmid p205880-3
Accession NZ_CP030305
Organism Klebsiella pneumoniae strain 205880

Toxin (Protein)


Gene name KacT Uniprot ID W8E895
Locus tag DRB04_RS27525 Protein ID WP_016479963.1
Coordinates 30256..30786 (-) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag DRB04_RS27530 Protein ID WP_022652312.1
Coordinates 30790..31059 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB04_RS27495 (DRB04_28365) 25471..25761 + 291 WP_004201172.1 co-chaperone GroES -
DRB04_RS27500 (DRB04_28370) 25817..27457 + 1641 WP_004201176.1 chaperonin GroEL -
DRB04_RS27505 (DRB04_28375) 27646..27828 + 183 WP_016479964.1 hypothetical protein -
DRB04_RS27510 (DRB04_28380) 28083..28202 + 120 Protein_38 IS30 family transposase -
DRB04_RS27515 (DRB04_28385) 28215..28961 + 747 Protein_39 IS3 family transposase -
DRB04_RS27520 (DRB04_28390) 29108..30088 - 981 WP_000019403.1 IS5-like element IS5 family transposase -
DRB04_RS27525 (DRB04_28400) 30256..30786 - 531 WP_016479963.1 GNAT family N-acetyltransferase Toxin
DRB04_RS27530 (DRB04_28405) 30790..31059 - 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
DRB04_RS27535 (DRB04_28410) 31915..32904 + 990 WP_022652311.1 RepB family plasmid replication initiator protein -
DRB04_RS27540 (DRB04_28415) 33200..33939 - 740 Protein_44 site-specific integrase -
DRB04_RS27545 (DRB04_28420) 34457..34573 + 117 Protein_45 IS5 family transposase -
DRB04_RS27550 (DRB04_28430) 34645..35765 + 1121 WP_089046468.1 IS3-like element ISSen4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul1 / rmtC / blaNDM-1 htpB 1..110786 110786
- inside IScluster/Tn - - 28296..56490 28194


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20350.24 Da        Isoelectric Point: 6.4907

>T107582 WP_016479963.1 NZ_CP030305:c30786-30256 [Klebsiella pneumoniae]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTREDKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T107582 NZ_CP030305:c30786-30256 [Klebsiella pneumoniae]
ATGGAGGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATGAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTTACTGACCATCTGAAACGGCAGCATAACAGTAAGATCTTACGTGGTTATGTTCTCGTGACGAGAGAGG
ATAAACCGCGTGTGATGGGGTACTACACGCTATCAGGAAGTTGCTTTGAGAAAATACTTCTACCCAGCAAAACACAACAA
AAGCGTGTACCCTACAAGAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCACCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AAGCGCTGAACGACAACGCCAAAAAATTCTACCTTCGACTGGGTTTCATTCAGTTAAAGGAAGAAAACTGTAACTCGCTC
TTTTACCCGACAAAATCTATTGAAGAATTGTTCGAAGTAAATGATGAATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT107582 WP_022652312.1 NZ_CP030305:c31059-30790 [Klebsiella pneumoniae]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT107582 NZ_CP030305:c31059-30790 [Klebsiella pneumoniae]
ATGTCAGCACTGAAAAAGCAGCGCATTGACCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCAATTTCGCAGTTCATGGTCAGTACCGCCTCAGAACGCGCTGCAGAAGTTATTGACCAGCACCGTCGTC
TGCTCCTCAATGAGGAGTCTTGGAACCTGGTTATGGATGCAATCATTAATCCGCCAGCACCGAATGACCGGCTGAAACGC
GCCGCTAACCGTCTCCGGGAACTGGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6C0L7Q2

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References