Detailed information of TA system TA107580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 104683..105326
• Replicon: plasmid p205880-1
• Accession: NZ_CP030303
• Organism: Klebsiella pneumoniae strain 205880

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: DRB04_RS25790
• Protein ID: WP_016236302.1
• Coordinates: 104683..105099 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: DRB04_RS25795
• Protein ID: WP_001261282.1
• Coordinates: 105096..105326 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB04_RS25770 (DRB04_26470)	100051..100401	+	351	WP_004187110.1	DUF305 domain-containing protein	-
DRB04_RS25775 (DRB04_26475)	100587..101495	-	909	WP_032439686.1	HNH endonuclease	-
DRB04_RS25780 (DRB04_26480)	102040..103062	-	1023	WP_032439672.1	helicase UvrD	-
DRB04_RS25785 (DRB04_26485)	103047..104609	-	1563	WP_004206609.1	AAA family ATPase	-
DRB04_RS25790 (DRB04_26490)	104683..105099	-	417	WP_016236302.1	type II toxin-antitoxin system VapC family toxin	Toxin
DRB04_RS25795 (DRB04_26495)	105096..105326	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DRB04_RS25800 (DRB04_26500)	105283..105705	+	423	WP_050485703.1	hypothetical protein	-
DRB04_RS25805 (DRB04_26505)	105905..106849	+	945	WP_032448293.1	hypothetical protein	-
DRB04_RS25810 (DRB04_26510)	106958..107350	+	393	WP_074422814.1	hypothetical protein	-
DRB04_RS25815 (DRB04_26515)	107408..107929	+	522	WP_032448294.1	hypothetical protein	-
DRB04_RS25820 (DRB04_26520)	107975..108760	+	786	WP_050485696.1	site-specific integrase	-
DRB04_RS25825	108880..109095	-	216	WP_129586912.1	hypothetical protein	-
DRB04_RS25830 (DRB04_26525)	109142..110317	-	1176	Protein_113	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA	1..229479	229479

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15091.55 Da
• Isoelectric Point: 7.1084

>T107580 WP_016236302.1 NZ_CP030303:c105099-104683 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T107580 NZ_CP030303:c105099-104683 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT107580 WP_001261282.1 NZ_CP030303:c105326-105096 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT107580 NZ_CP030303:c105326-105096 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAAGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GZG3