Detailed information of TA system TA107579

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 59682..60418
• Replicon: plasmid p205880-1
• Accession: NZ_CP030303
• Organism: Klebsiella pneumoniae strain 205880

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: DRB04_RS25565
• Protein ID: WP_004187044.1
• Coordinates: 59682..60164 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: DRB04_RS25570
• Protein ID: WP_003026799.1
• Coordinates: 60152..60418 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB04_RS25535 (DRB04_26235)	54768..54917	+	150	Protein_54	transposase	-
DRB04_RS25540	54933..55043	+	111	Protein_55	IS3 family transposase	-
DRB04_RS25545 (DRB04_26240)	55191..55589	-	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
DRB04_RS25550 (DRB04_26245)	55638..56984	-	1347	WP_077254728.1	ISNCY family transposase	-
DRB04_RS25555 (DRB04_26250)	57261..57965	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
DRB04_RS25560 (DRB04_26255)	58125..59471	-	1347	WP_077251107.1	ISNCY family transposase	-
DRB04_RS25565 (DRB04_26260)	59682..60164	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
DRB04_RS25570 (DRB04_26265)	60152..60418	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
DRB04_RS25575 (DRB04_26275)	61530..62339	-	810	WP_023329017.1	N-formylglutamate deformylase	-
DRB04_RS25580 (DRB04_26280)	62332..63540	-	1209	WP_032448288.1	imidazolonepropionase	-
DRB04_RS25585 (DRB04_26285)	63552..64946	-	1395	WP_032439652.1	cytosine permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA	1..229479	229479

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T107579 WP_004187044.1 NZ_CP030303:c60164-59682 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T107579 NZ_CP030303:c60164-59682 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTTATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT107579 WP_003026799.1 NZ_CP030303:c60418-60152 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT107579 NZ_CP030303:c60418-60152 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66