Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 127054..127579 Replicon plasmid p283747-1
Accession NZ_CP030301
Organism Klebsiella pneumoniae strain 283747

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag DRB02_RS27955 Protein ID WP_013023785.1
Coordinates 127274..127579 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag DRB02_RS27950 Protein ID WP_001568025.1
Coordinates 127054..127272 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB02_RS27925 (123180) 123180..124202 - 1023 WP_000361404.1 helicase UvrD -
DRB02_RS27930 (124187) 124187..125749 - 1563 WP_004206609.1 AAA family ATPase -
DRB02_RS27935 (125823) 125823..126239 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
DRB02_RS27940 (126236) 126236..126466 - 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin -
DRB02_RS27945 (126423) 126423..126884 + 462 WP_160866775.1 hypothetical protein -
DRB02_RS27950 (127054) 127054..127272 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
DRB02_RS27955 (127274) 127274..127579 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
DRB02_RS27960 (127748) 127748..128143 + 396 WP_017899885.1 hypothetical protein -
DRB02_RS27965 (128170) 128170..128484 + 315 WP_053389906.1 hypothetical protein -
DRB02_RS27970 (128495) 128495..129511 + 1017 WP_017899884.1 hypothetical protein -
DRB02_RS27975 (129709) 129709..130503 + 795 WP_004197635.1 site-specific integrase -
DRB02_RS28345 (130975) 130975..131277 - 303 WP_004197636.1 hypothetical protein -
DRB02_RS27985 (131274) 131274..131900 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..148021 148021


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T107562 WP_013023785.1 NZ_CP030301:127274-127579 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T107562 NZ_CP030301:127274-127579 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT107562 WP_001568025.1 NZ_CP030301:127054-127272 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT107562 NZ_CP030301:127054-127272 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References