Detailed information of TA system TA107561

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 125823..126466
• Replicon: plasmid p283747-1
• Accession: NZ_CP030301
• Organism: Klebsiella pneumoniae strain 283747

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: DRB02_RS27935
• Protein ID: WP_001044770.1
• Coordinates: 125823..126239 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: DRB02_RS27940
• Protein ID: WP_001261282.1
• Coordinates: 126236..126466 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DRB02_RS27915 (121926)	121926..122198	-	273	Protein_168	transposase	-
DRB02_RS27925 (123180)	123180..124202	-	1023	WP_000361404.1	helicase UvrD	-
DRB02_RS27930 (124187)	124187..125749	-	1563	WP_004206609.1	AAA family ATPase	-
DRB02_RS27935 (125823)	125823..126239	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
DRB02_RS27940 (126236)	126236..126466	-	231	WP_001261282.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
DRB02_RS27945 (126423)	126423..126884	+	462	WP_160866775.1	hypothetical protein	-
DRB02_RS27950 (127054)	127054..127272	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
DRB02_RS27955 (127274)	127274..127579	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
DRB02_RS27960 (127748)	127748..128143	+	396	WP_017899885.1	hypothetical protein	-
DRB02_RS27965 (128170)	128170..128484	+	315	WP_053389906.1	hypothetical protein	-
DRB02_RS27970 (128495)	128495..129511	+	1017	WP_017899884.1	hypothetical protein	-
DRB02_RS27975 (129709)	129709..130503	+	795	WP_004197635.1	site-specific integrase	-
DRB02_RS28345 (130975)	130975..131277	-	303	WP_004197636.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65	-	1..148021	148021

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T107561 WP_001044770.1 NZ_CP030301:c126239-125823 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T107561 NZ_CP030301:c126239-125823 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT107561 WP_001261282.1 NZ_CP030301:c126466-126236 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT107561 NZ_CP030301:c126466-126236 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3