Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5422409..5423034 Replicon chromosome
Accession NZ_CP030300
Organism Klebsiella pneumoniae strain 283747

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag DRB02_RS26735 Protein ID WP_002882817.1
Coordinates 5422409..5422792 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag DRB02_RS26740 Protein ID WP_004150355.1
Coordinates 5422792..5423034 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB02_RS26720 (DRB02_27575) 5419775..5420677 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
DRB02_RS26725 (DRB02_27580) 5420674..5421309 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
DRB02_RS26730 (DRB02_27585) 5421306..5422235 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
DRB02_RS26735 (DRB02_27590) 5422409..5422792 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
DRB02_RS26740 (DRB02_27595) 5422792..5423034 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
DRB02_RS26745 (DRB02_27600) 5423239..5424156 + 918 WP_002882812.1 alpha/beta hydrolase -
DRB02_RS26750 (DRB02_27605) 5424170..5425111 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
DRB02_RS26755 (DRB02_27610) 5425156..5425593 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
DRB02_RS26760 (DRB02_27615) 5425590..5426450 - 861 WP_002882807.1 virulence factor BrkB family protein -
DRB02_RS26765 (DRB02_27620) 5426444..5427043 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T107553 WP_002882817.1 NZ_CP030300:c5422792-5422409 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T107553 NZ_CP030300:c5422792-5422409 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT107553 WP_004150355.1 NZ_CP030300:c5423034-5422792 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT107553 NZ_CP030300:c5423034-5422792 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References