Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4223005..4223624 Replicon chromosome
Accession NZ_CP030300
Organism Klebsiella pneumoniae strain 283747

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag DRB02_RS20990 Protein ID WP_002892050.1
Coordinates 4223406..4223624 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag DRB02_RS20985 Protein ID WP_002892066.1
Coordinates 4223005..4223379 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DRB02_RS20975 (DRB02_21560) 4218157..4219350 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DRB02_RS20980 (DRB02_21565) 4219373..4222519 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
DRB02_RS20985 (DRB02_21570) 4223005..4223379 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
DRB02_RS20990 (DRB02_21575) 4223406..4223624 + 219 WP_002892050.1 HHA domain-containing protein Toxin
DRB02_RS20995 (DRB02_21580) 4223783..4224349 + 567 WP_002892030.1 maltose O-acetyltransferase -
DRB02_RS21000 4224321..4224461 - 141 WP_004147370.1 hypothetical protein -
DRB02_RS21005 (DRB02_21585) 4224482..4224952 + 471 WP_002892026.1 YlaC family protein -
DRB02_RS21010 (DRB02_21590) 4224927..4226378 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
DRB02_RS21015 (DRB02_21595) 4226479..4227177 + 699 WP_002892021.1 GNAT family protein -
DRB02_RS21020 (DRB02_21600) 4227174..4227314 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
DRB02_RS21025 (DRB02_21605) 4227314..4227577 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T107549 WP_002892050.1 NZ_CP030300:4223406-4223624 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T107549 NZ_CP030300:4223406-4223624 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT107549 WP_002892066.1 NZ_CP030300:4223005-4223379 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT107549 NZ_CP030300:4223005-4223379 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References