Detailed information of TA system TA107530

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1770..2317
• Replicon: plasmid p309074-2
• Accession: NZ_CP030298
• Organism: Klebsiella pneumoniae strain 309074

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: DQG43_RS26780
• Protein ID: WP_244493824.1
• Coordinates: 2039..2317 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6G9U792
• Locus tag: DQG43_RS26775
• Protein ID: WP_000079935.1
• Coordinates: 1770..2039 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DQG43_RS26770 (1)	1..858	+	858	WP_016240488.1	incFII family plasmid replication initiator RepA	-
DQG43_RS26775 (1770)	1770..2039	+	270	WP_000079935.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
DQG43_RS26780 (2039)	2039..2317	+	279	WP_244493824.1	type II toxin-antitoxin system toxin YacB	Toxin
DQG43_RS26785 (2363)	2363..3211	+	849	WP_001058003.1	3'-5' exonuclease	-
DQG43_RS26790 (3328)	3328..3810	+	483	WP_001311056.1	hypothetical protein	-
DQG43_RS26795 (4170)	4170..5286	-	1117	Protein_5	IS91 family transposase	-
DQG43_RS26800 (5286)	5286..5651	-	366	WP_000124093.1	hypothetical protein	-
DQG43_RS26805 (6179)	6179..6688	+	510	WP_000115001.1	peptide deformylase	-
DQG43_RS26810 (6703)	6703..6804	+	102	Protein_8	methionyl-tRNA formyltransferase	-
DQG43_RS26815 (7001)	7001..7192	+	192	WP_228275525.1	DUF2164 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..70438	70438
	-	flank	IS/Tn	-	-	4201..4923	722

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10729.37 Da
• Isoelectric Point: 8.4615

>T107530 WP_244493824.1 NZ_CP030298:2039-2317 [Klebsiella pneumoniae]
MEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYSASSLAGLPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T107530 NZ_CP030298:2039-2317 [Klebsiella pneumoniae]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATAGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCTGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10198.53 Da
• Isoelectric Point: 8.8735

>AT107530 WP_000079935.1 NZ_CP030298:1770-2039 [Klebsiella pneumoniae]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLTHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT107530 NZ_CP030298:1770-2039 [Klebsiella pneumoniae]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTACCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6G9U792